Isomeric Sugar Recognition Using Molecularly Imprinted Polymer Hydrogels

摘要题目：分子印迹聚合物水凝胶识别糖异构体建议编号：CTS-0303951首席研究员：彼得·科菲纳斯研究所：马里兰大学拟议研究的目的是制备和测试具有不同材料识别性能的分子印迹聚合物水凝胶。分子印迹聚合物(MIP)将被合成并针对特定的简单糖异构体进行印迹。将开发检测和量化每个分子印迹的方法。MIP的总糖结合力、特异性和糖扩散速率将在水和生理介质中进行评估。特定的糖识别将通过组合合成方法进行优化。将在水溶液中进行非共价印迹方法。机理研究将试图阐明分子形状和官能度对选择性的作用。就更广泛的影响而言，新型分子印迹聚合物可能为生物识别和异构体分离技术提供新的途径。本研究开发的分子印迹聚合物可与层析技术结合用于生物分离，以分离氨基酸、糖、药物、病毒和其他生物活性化合物。在以前的工作中，已经制定了K-12活动。在这个项目中，将为K-12开发一个互动网站。

AbstractProposal Title: Isomeric Sugar Recognition Using Molecularly Imprinted Polymer HydrogelsProposal Number: CTS-0303951Principal Investigator: Peter KofinasInstitution: University of MarylandThe objective of the proposed research is to prepare and test polymer hydrogels that have been molecularly imprinted with recognition properties for different materials. Molecularly imprinted polymers (MIP) will be synthesized and imprinted against specific simple sugar isomers. Methods will be developed for the detection and quantification of each molecular imprint. The MIP's total sugar binding, specificity, and rate of sugar diffusion will be evaluated in aqueous and physiological media. Specific sugar recognition will be optimized through combinatorial synthesis methods. A non-covalent approach to imprinting will be carried out in aqueous solution. Mechanistic studies will attempt to clarify the roles of molecular shape and functionality on selectivity. In terms of the broader impacts, novel MIPs might offer new avenues for biorecognition and isomeric separation techniques. The MIPs developed in this study may be used for bio-separations in conjunction with chromatographic techniques to separate amino acids, sugars, drugs, viruses and other bioactive compounds. In previous work K-12 activities have been developed. An interactive Website for K-12 will be developed in this project.