Capillary Electrophoresis in the Undergraduate Curriculum in Biochemistry and Forensic Chemistry

化学（12） 毛细管电泳（CE）技术已被科学界广泛接受。为了让学生为科学职业或追求高级学位做好充分准备，我们将CE方法纳入我们的本科课程。薄层层析，SDS-聚丙烯酰胺和琼脂糖凝胶电泳，和传统的液相色谱法已被用于分离生物分子在我们的本科生生物化学实验室一段时间。随着一个多功能的CE系统，可用于多种类型的分子的分析收购，我们现在正在调整和实施一套专门设计的实验到我们的本科生物化学和法医化学课程。这些实验和协议都是改编自教育和研究期刊，以及NSF支持的项目，这些项目已经过全面测试。这些选定的实验使我们的学生能够对生物学，药学和法医学意义的样品进行定性和定量分析（例如，蛋白质、DNA和药物分析）。我们还在执法和司法行政、生物和化学部门之间开展合作，使用CE方法。在成功地将CE纳入生物化学和法医化学课程的预期，我们计划扩大CE系统的应用一般，有机，分析，物理和无机化学课程。CE系统也用于我们系的本科研究。我们计划通过在适当的期刊上发表文章、参加科学会议和为该项目建立一个网站来传播该项目的成果。

Chemistry (12) Capillary electrophoresis (CE) techniques have become widely accepted by the scientific community. To fully prepare students for careers in science or to pursuit advanced degree, we are including CE methodology into our undergraduate curriculum. Thin layer chromatography, SDS-polyacrylamide and agarose gel electrophoresis, and conventional liquid chromatography have been used for some time to separate biomolecules in our undergraduate biochemistry laboratories. With the acquisition of a versatile CE system that can be used in the analysis of many types of molecules, we are now adapting and implementing a specially designed set of experiments into our undergraduate biochemistry and forensic chemistry curriculum. The experiments and protocols are all adapted from educational and research journals, and NSF supported projects that have been fully tested. These selected experiments allow our students to perform qualitative and quantitative analysis of samples of biological, pharmaceutical, and forensic significance (e.g., protein, DNA, and drug analysis) by direct comparison of CE to other methods of instrumental separation. We are also developing collaborations between the departments of law enforcement and justice administration, biology, and chemistry on the use of CE methodology. After successful incorporation of CE in biochemistry and forensic chemistry courses as anticipated, we plan to expand the applications of the CE system to general, organic, analytical, physical, and inorganic chemistry courses. The CE system is also used in undergraduate research in our department. We plan to disseminate the outcome of this project through publications in appropriate journals, attending scientific meetings, and establishing a web site for the project.