Arabidopsis 2010: Arabidopsis Polyadenylation Factor Subunits - Mutants and Protein Interaction Networks

信使RNA（mRNAs）的多聚腺苷酸化是植物基因表达过程中的一个重要步骤。 多聚腺苷酸化为mRNA添加了一个重要元素，并有助于协调信使RNA的合成及其在蛋白质合成中的最终功能。 因此，组成多聚腺苷酸化机制的蛋白质亚基有望在植物生长中发挥重要作用。 该项目的目标是确定编码聚腺苷酸化机制亚基的拟南芥基因组的集体功能（该基因组，包括拟南芥基因组标识符，可以在http://www.uky.edu/~aghunt00/polyA2010.html和http：//www.users.muohio.edu/liq/arabidopsis2010.html上找到）。 这将包括一个文件的表型的植物，有一个或多个亚基删除（通过突变或基因沉默），并确定一套完整的蛋白质-蛋白质相互作用，涉及各种亚基。 结果将通过同行评审出版物以及主要研究者管理的网页（http：//www.example.com和http：//www.users.muohio.edu/liq/arabidopsis2010.html）传播。www.uky.edu/~aghunt00/polyA2010.html 此外，突变体拟南芥原种将存放在适当的原种中心。这项研究将有助于实现2010年项目的一个目标（“到2010年确定参考物种的所有基因在其细胞、生物体和进化背景中的功能”），通过对突变体表型进行编目，通过定义拟南芥多聚腺苷酸化因子亚基之间的相互作用网络，通过鉴定与多聚腺苷酸化机制相互作用的功能未知的蛋白质，将大大增加目前对高等植物mRNA 3'末端形成的理解。 因此，它将提高定制外源基因在转基因植物中表达的能力，从而为农民开发改良作物（以及新的替代经济机会）。 这也将为mRNA多聚腺苷酸化的进化提供重要的见解。 研究产品（克隆cDNA、抗体、植物品系等）将提供给整个研究界。 该研究将有助于培养两名博士后学者，四名研究生和多达十二名本科生。 后者将从肯塔基州大学和迈阿密大学备受推崇的多样化课程中挑选。

The polyadenylation of messenger RNAs (mRNAs) is an important step in the process of gene expression in plants. Polyadenylation adds an essential element to the mRNA and helps to coordinate the synthesis of a messenger RNA with its ultimate functioning in protein synthesis. Consequently, the protein subunits that make up the polyadenylation machinery are expected to play important roles in plant growth. The goals of this project are to determine the collective function of the set of Arabidopsis genes that encode subunits of the polyadenylation machinery (this set, including Arabidopsis genome identifiers, can be found at http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). This will include a documentation of the phenotypes of plants that have had one or more subunit removed (by mutation or gene silencing) and a determination of the complete set of protein-protein interactions involving the various subunits. The results will be disseminated via peer-reviewed publication as well as through web pages managed by the principal investigators (http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). In addition, mutant Arabidopsis stocks will be deposited with the appropriate stock center(s). The research will contribute to a goal of the 2010 Project ("to determine the function of all genes of a reference species within their cellular, organismal and evolutionary contexts by the year 2010") by cataloguing mutant phenotypes, by defining the network of interactions amongst Arabidopsis polyadenylation factor subunits, and by identifying proteins of unknown function that interact with the polyadenylation machinery.The research will greatly increase the current understanding of mRNA 3' end formation in higher plants. As such, it will enhance the ability to tailor foreign genes for expression in transgenic plants, and thereby develop improved crops (as well as novel alternative economic opportunities) for farmers. It will also lend significant insight into the evolution of mRNA polyadenylation. The research products (cloned cDNA, antibodies, plant lines etc.) will be made available to the research community at large. The research will contribute to the training of two postdoctoral scholars, four graduate students, and as many as twelve undergraduates. The latter will be drawn from highly-regarded and diverse programs at the University of Kentucky and Miami University.