SBIR Phase I: Detection and Identification Instrument for Single Molecule Analysis

这个小型企业创新研究（SBIR）第一阶段项目提出开发一种用于遗传分析和单分子研究的新型低成本实验室仪器。该技术适用于通过荧光杂交探针检测和鉴定DNA和RNA，而无需聚合酶链反应扩增，或通过荧光免疫测定法检测和鉴定蛋白质和小分子。一般方案是基于单分子检测（SMD），并利用双色互相关光谱（TC-FCCS）技术与符合检测分析方案，同时探测微流控测定的10个焦点区域。高效率的单光子探测率的盖革模式微雪崩光电二极管（uAPD）阵列将作为探测元件，其商业应用将在研究、医学应用和药物开发中发挥作用。应用范围从构象动力学和大分子相互作用的研究到单分子的生化动力学。

This Small Business Innovative Research (SBIR) Phase I project proposes to develop a novel, low cost laboratory instrument for genetic analysis and single molecule studies. The technology is suitable for the detection and identification of DNA and RNA through fluorescent hybridization probes without the need for Polymerase Chain Reaction amplification, or for proteins and small molecules through fluorescence immunoassays. The general scheme is based on single molecule detection (SMD) and utilizes the two-color cross-correlation spectroscopy (TC-FCCS) technique with coincident detection analysis scheme to simultaneously probe ten focal regions of a microfluidic assay. High efficiency single photon detectivity Geiger mode microavalanche photodiode (uAPD) arrays will function as detection elements.The commercial applications of the instrument will be in research, medical applications, and for drug development. Applications range from the study of conformational dynamics and interactions of macromolecules to biochemical kinetics of single molecules.