SENSORS: Electrochemical Impedance Architecture for Biosensors

本项目的目的是开发一种新的生物传感检测方案。这包括电化学阻抗谱（EIS）的生物传感器转导和受体蛋白基因工程的表面固定化。EIS可以探测生物传感器界面上的电荷转移和电容，比竞争对手只涉及安培检测的方法提供更多的信息。将探讨这种方法在小分子配体结合过程中使用受体蛋白进行大振幅运动的一般适用性。这种EIS检测方案与定向固定基因工程蛋白到金电极表面的方法相结合。本研究的最终目标是开发用于检测生物兴趣的大小配体和底物的一般方法。就更广泛的影响而言，临床诊断、农业、国防和制药行业可能会受益。该项目将在一个高度跨学科的领域为学生提供研究生教育和培训，这项研究的结果将在互联网上传播。

The objective of this project is to develop a new detection scheme for biosensing. This involves both biosensor transduction by electrochemical impedance spectroscopy (EIS) and receptor protein genetic engineering for surface immobilization. EIS can probe both charge transfer and capacitance at the biosensor interface, providing more information than competing methods that involve only amperometric detection. The general applicability of this approach using receptor proteins that undergo large amplitude motion during small-molecule ligand binding will be explored. This EIS detection scheme is coupled to the development of methods for oriented immobilization of genetically engineered proteins onto a gold electrode surface. The ultimate goal of this research is the development of general methods for detection of both small and large ligands and substrates of biological interest. In terms of the broader impacts, the areas of clinical diagnostics, agriculture, national defense, and the drug industries may benefit. The project will provide graduate education and training for students in a highly interdisciplinary field, and the results of this research will be disseminated over the Internet.