Application of Different Methodologies to Identify Sources of Fecal Pollution in The Rio Grande River

美国--墨西哥边境地区有着与水资源有关的独特问题。 格兰德河/布拉沃河是两国区域的主要分水岭。 这条河是两国工业、农业、生活用水供应、娱乐和野生动物栖息地的重要自然资源。 不幸的是，格兰德河也是传染性微生物和有毒污染物的通道。 已经确定了导致水源水化学和微生物污染的各种活动，包括不正确安装和维护的化粪池系统、垃圾填埋场、注入威尔斯井、废物的土地应用、灌溉、径流和动物饲料场。 该项目是埃尔帕索县社区学院和亚利桑那州立大学水质中心之间的合作努力。 本项目的目的是进行一项全面的比较研究，以确定格兰德河四个地点的粪便污染源，使用1）粪链球菌的抗生素耐药性分析，2）大肠杆菌的抗生素耐药性分析，3）选择的大肠杆菌的核糖分型。4）大肠杆菌的基因分型; coli和F-RNA噬菌体。

The U.S.-Mexico Border region has unique problems related to water resources. The Rio Grande/Rio Bravo is the major watershed of the bi-national region. The river serves as an important natural resource for industry, agriculture, domestic water supply, recreation, and wildlife habitat fro both countries. Unfortunately, the Rio Grande is also a conduit for infectious microorganisms and toxic pollutants. A variety of activities contributing to the chemical and microbial contamination of source water have been identified and include improperly installed and maintained septic systems, landfill, injection wells, land application of waste, irrigation, runoff, and animal feed lots. This project is a collaborative effort between El Paso County Community College and the Water Quality Center at Arizona State University. The objective of this project is to conduct a comprehensive and comparative study to determine the source of fecal contamination at four sites of the Rio Grande river using 1) Antibiotic Resistance Analysis of fecal streptococci, 2) Antibiotic Resistance Analysis of E coli, 3) Ribotyping of selected E. coli isolates, and 4) Genotyping of E. coli and F-RNA bacteriophages.