Neuro Imaging through Internet2

西奈山医学院（Mount Sinai School of Medicine， MSSM）正在为Abilene/Internet2寻求资金，以更好地处理图像分析和处理的需求。最近国家研究资源中心（NCRR）的拨款允许该机构升级主研究大楼的计算机网络。广域网现在成为与其他机构合作的瓶颈。该连接本身也将支持以下领域的合作项目：哺乳动物神经元的三维形态测量分析：MSSM与来自Courant数学科学研究所（NYU）、纽约大学医学院、纽约州立大学石溪分校和澳大利亚新南威尔士大学的研究人员合作。MSSM的研究人员研究大脑中神经元的结构-功能关系，采用先进的3D显微成像技术以及3D图像处理，渲染，分析和建模。CNIC的研究人员正在使用共聚焦和多光子显微镜以尽可能高的分辨率对皮质中的整个锥体神经元进行三维成像。这为单个神经元创建了大约20g的数据集。数据采集完成后，导出到计算机中，在计算机中进行图像拼接、体绘制、反卷积和3d图像分析。该合同将极大地促进非现场合作者访问这些大型数据集的能力，以及使用由CNIC研究人员开发并在MSSM托管的独特软件操作和分析数据。前庭功能的神经机制：来自华盛顿大学、犹他大学和NASA-Ames的MSSM研究人员和合作者正在研究前庭功能。正在进行电生理和显微镜研究，以确定传入反应动力学在前庭感觉上皮的区域变化的基础。600兆字节到1.2千兆字节的数据集是使用多光激光器创建的。通过访问承载在MSSM计算机上的体绘制和3D图像分析软件来操作3D数据的能力将为非现场合作者提供宝贵的信息。此外，由于任何一个实验都需要很长的时间来收集数据，因此合作者在多光子显微镜上实时收集数据时将非常有利于审查数据。这将避免反馈的任何延迟，并允许非现场合作者修改实验条件。

Mount Sinai School of Medicine (MSSM) seeks funding for an Abilene/Internet2 to better handle the demands of image analysis and processing. A recent National Center for Research Resources (NCRR) grant allowed the institution to upgrade the computer network in the main research building. The wide area network now presents a bottleneck for collaboration with other institutions. The connection, itself, will also support collaborative projects in the following areas: 3-dimensional Morphometric Analysis of Mammalian Neurons: A collaboration between MSSM, investigators from the Courant Institute of Mathematical Sciences (NYU), New York University School of Medicine, SUNY at Stony Brook, and the University of New South Wales, Australia. Researchers at MSSM study the structure-function relationship of neurons in the brain, employing advanced 3D microscopic imaging techniques as well as 3D image processing, rendering, analysis and modeling. CNIC investigators are using confocal and multi-photon microscopes to image (in 3D) entire pyramidal neurons in the cortex at the highest resolution possible. This creates approximately 20-gigabyte data sets for a single neuron. After the data is collected, it is exported to computers, where image stitching, volume rendering, deconvolution and 3D-image analysis is done. This award will greatly facilitate the capabilities of off-site collaborators to access these large datasets as well as manipulate and analyze the data using unique software developed by CNIC investigators and hosted at MSSM. Neural Mechanisms of Vestibular Function: MSSM researchers and collaborators at Washington University, the University of Utah and NASA-Ames are studying vestibular functions. Electrophysiological and microscopic studies are being performed to determine the basis for regional variations in afferent response dynamics across the vestibular sensory epithelium. 600 Mbytes to 1.2-gigabyte data sets are created using a multi-photo laser. The ability to manipulate the data in 3D via access to volume rendering and 3D image analysis software hosted on MSSM computers will provide invaluable information to off-site collaborators. Furthermore, due to the length of time required to compile the data for any one experiment, it will be greatly beneficial for collaborators to review the data as it is collected (in real time) on the multi-photon microscope. This will avoid any delay in feedback and permit the off-site collaborators to modify experimental conditions.