Protein Adsorption and Transport in Chromatographic Bioprocessing

摘要提案标题：蛋白质在层析生物处理中的吸附和转运提案号：CTS-0350631主要研究者：Abraham Lenhoff机构：特拉华大学本项目的目的是确定蛋白质在层析分离中的吸附和转运机制。 一个目标是开发蛋白质和固定相的结构性质之间的定量关系。 色谱性能将根据线性保留、容量和颗粒内转运进行表征。这些措施与固定相、蛋白质和洗脱液的基本物理、化学和结构性质有关。研究将集中在两种模式的色谱：离子交换色谱（IEC）和疏水相互作用色谱（HIC）。 还将测定蛋白质吸附动力学。 就更广泛的影响而言，色谱法广泛用于生物加工的下游纯化。 拟议的研究可以提供可用于指导分离过程的定量机理模型。 学生的培训将涵盖从现代实验工具，如共聚焦显微镜到生物加工中的色谱实践。

AbstractProposal Title: Protein Adsorption and Transport in Chromatographic BioprocessingProposal Number: CTS-0350631Principal Investigator: Abraham LenhoffInstitution: University of DelawareThe objective of this project is to determine the mechanisms of adsorption and transport in chromatographic separations of proteins. One goal is to develop quantitative relationships between structural properties of the protein and the stationary phase. Chromatographic performance will be characterized in terms of linear retention, capacity, and intraparticle transport. These measures are related to the underlying physical, chemical and structural properties of the stationary phase, the protein, and the eluent. Research will focus on two modes of chromatography: ion exchange chromatography (IEC) and hydrophobic interaction chromatography (HIC). Protein adsorption kinetics will also be determined. In terms of the broader impacts, chromatography is widely used in downstream purification in bioprocessing. The proposed studies can provide quantitative mechanistic models that can be used to guide separation processes. Student training will span a spectrum from modern experimental tools such as confocal microscopy to the practice of chromatography in bioprocessing.