Acquisition and testing of the ImageStream 100 (beta model): Improving quality and expanding the scope of the Integrated Primate Biomaterial and Information Resource.

在国家科学基金会重大研究仪器奖的支持下，Robert Hanner博士和他在Coriell医学研究所和Drexel大学的同事将为贴壁细胞和流动细胞购买免费的最先进的细胞成像系统。该研究所以前的成像技术缺乏NSF资助的综合灵长类动物生物材料和信息资源（IPBIR）的发展所需的分辨率和高通量能力，该资源在开发培养各种非人类灵长类动物组织所需的新协议方面面临障碍。流动细胞成像的新技术，与贴壁细胞的高通量机器人共聚焦显微镜互补，将推进灵长类动物组织培养和IPBIR中罕见和难以获得的样品的细胞表征的最新技术水平。与细胞生物学家团队合作，汉纳博士和他的同事PI Jeanne Beck博士将在许多研究中监督和管理该系统，这将有助于检查培养细胞的结构并优化最佳要求。为了他们的文化。这些系统将为大量研究人员提供高内容筛选能力。高含量筛选（HCS）和定量荧光显微镜已经彻底改变了细胞生物学。 HCS被定义为阵列细胞的细胞生物学研究的自动化，包括实验设计、样品制备、图像采集、存档、处理和分析以及细胞知识挖掘等关键操作。短语“高含量”是指在细胞阵列内的每个单独细胞的结构和功能完整性的背景下在空间和时间上定义的过程。这个新兴的细胞成像卓越中心的一个关键特点是能够产生定量数据，使仪器能够用于广泛的科学调查，从仪器获得的数据将作为IPBIR目录的一部分提供，记录从2005年开始由资源提供给科学界的生物材料。 该目录（www.ipbir.org）将向研究人员、教育工作者和其他对收集的数据感兴趣的人提供原始数据和处理后的数据。原始数据可用于涉及“系统生物学”方法下的数据挖掘的科学研究。以个人高分辨率图像的形式处理的数据将使教育工作者能够向学生教授细胞形态学的基础知识，并提供细胞表达和分化模式的真实的例子。IPBIR目录由Coriell医学研究所维护和支持。这个项目很重要，原因有几个。它将为研究人员提供一流的高通量细胞成像能力，该研究机构具有进行尖端细胞培养和表征的能力。该系统还将通过积极参与德雷克塞尔大学生物信息学项目的研究生以及罗格斯大学、罗文大学和威尔逊学院的本科生，在培养下一代科学家方面发挥重要作用。涉及该仪器的研究将确保已投资于IPBIR的基础设施的最佳开发和使用，促进全球高度特征化的灵长类动物细胞的可用性。

With support from a National Science Foundation Major Research Instrumentation Award, Dr. Robert Hanner and his colleagues at the Coriell Institute for Medical Research and Drexel University will purchase complimentary state of the art cellular imaging systems for both adherent cells and cells in flow. Previous imaging techniques at the Institute lacked both the resolution and high throughput capabilities needed for the advancement of the NSF-funded Integrated Primate Biomaterials and Information Resource (IPBIR), which faces obstacles in developing new protocols needed to culture a diverse array of nonhuman primate tissues. New technologies for imaging cells in flow, complimented with high throughput robotic confocal microscopy of adherent cells, will advance the state of the art in primate tissue culture and cellular characterization of the rare and difficult to obtain samples in the IPBIR.Working with a team of cell biologists, Dr. Hanner and Co-PI Dr. Jeanne Beck will supervise and administer the systems in many studies that will help examine the structure of cultured cells and refine the optimal requirements for their culture. These systems will provide a large group of researchers with high content screening capabilities. High content screening (HCS) and quantitative fluorescence microscopy have revolutionized cell biology. HCS is defined as the automation of cell biological investigation of arrayed cells including key operations of experimental design, sample preparation, image acquisition, archiving, processing and analysis, and cellular knowledge mining. The phrase 'high content' refers to processes that are defined spatially and temporally in the context of the structural and functional integrity of each individual cell within an array of cells. A key feature of this emerging center of excellence for cellular imaging is the ability to produce quantitative data, allowing the instrument to be used for a wide range of scientific investigations.Data obtained from the instrumentation will be made available as part of the IPBIR catalog, documenting the biomaterials made available to the scientific community by the Resource, beginning in 2005. This catalog (www.ipbir.org) will provide both raw and processed data to researchers, educators, and others who are interested in the data that is gathered. The raw data could be used for scientific studies involving data mining under a 'systems biology' approach. The processed data, in the form of individual high-resolution images would enable educators to teach students basics of cellular morphology and provide real world examples of cellular expression and differentiation patterns. The IPBIR catalog is maintained and supported by the Coriell Institute for Medical Research.This project is important for several reasons. It will provide first-class high-throughput cellular imaging capabilities for researchers at an institution with demonstrated ability to conduct cutting-edge cell culture and characterization. The systems will also play a significant role in training the next generation of scientists by actively involving graduate students from Drexel University's Bioinformatics Program, as well as undergraduates from Rutgers, Rowan and Wilson Colleges. Research involving this instrumentation will ensure the optimal development and use of the infrastructure already invested into IPBIR, promoting the availability of highly characterized primate cells worldwide.