Sensors: Immobilization Technology for Array-based Biosensors

摘要表面等离子体共振生物传感器是分子间相互作用研究的一个革命。 目前的仪器可以提供详细的动力学信息复杂的形成没有试剂标记，但低通量。 新的基于阵列的生物传感器技术通过增加可以同时监测的表面反应的数量，为蛋白质组学革命做出了重大贡献。生产蛋白质阵列的挑战有两个方面：在表面上沉积和沉积活性蛋白质，以及在小的目标区域（通常为1平方厘米）内物理创建密集的蛋白质阵列。 目前的奖项旨在优化肽的固体针点样条件，并制造新的流体设备，将允许在流动蛋白质固定化。这项工作的更广泛的影响，包括培养学生在生物传感器技术和核受体生物学。此外，这项工作所产生的技术将允许蛋白质相互作用的大规模绘图，这将作为解释复杂生物系统的基础。

ABSTRACTSurface plasmon resonance biosensors have revolutionized the study ofmolecular interactions. Current instruments can provide detailed kineticinformation about complex formation without reagent labeling, but arelow throughput. New array-based biosensor technology is poised tomake a significant contribution to the proteomics revolution by increasing the number of surface reactions that can be monitored simultaneously. Thechallenges in producing protein arrays are two fold: depositing and maintainingactive proteins on a surface and physically creating dense arrays of proteins within small target areas (typically 1 cm2). The current award seeks to optimize the conditions for solid-pin spotting of peptides and to fabricate novel fluidic devices that would allow for in-flow protein immobilization.The broader impacts of this work include training students in biosensor technologyand nuclear receptor biology. In addition, the technology that results from this workwill permit large-scale mapping of protein interactions, which will serve as thebasis for interpreting complex biological systems.