SGER:Developing High Throughput Protein Network Analysis to Determine the Function of Unknown Rice Genes

该项目的目标是探索利用高通量蛋白质组学来确定基于细胞相互作用网络的编码蛋白质的基因功能。它适合用于探索性研究的小额赠款，因为它“冒险探索新兴的和潜在的变革性研究思想”。具体来说，将开发方法，通过蛋白质：蛋白质相互作用的水稻基因的未知功能可以确定。这些信息将允许识别哪些具有已知细胞作用的蛋白质与给定的未知蛋白质相互作用，从而将未知蛋白质与该细胞功能联系起来。这些信息将在一个公开可用的数据库中获取。这些数据还将允许构建基于广泛基础的水稻蛋白质相互作用图。将在基因组序列分析与蛋白质相互作用分析相结合的领域提供研究生培训。当社区努力整合这些不同的大型数据集时，这些技能将是至关重要的。此外，所开发的方法可以创造一个范式转变，这可能对定义分析复杂植物基因组的新策略产生重大影响。这项工作产生的数据将在：http://www.cshl.edu/genseq/riceproteome上提供。

The goal of this project is to explore the use of high-throughput proteomics to determine gene function based on the cellular interaction network of an encoded protein. It is appropriate for a Small Grant for Exploratory Research because it "ventures into emerging and potentially transformative research ideas". Specifically, methods will be developed by which the protein: protein interactions of rice genes of unknown function can be determined. This information will allow identification which protein(s) with known cellular role(s) interact with a given unknown protein, and will therefore will link the unknown protein to that cellular function. The information will be captured in a publicly available database. The data will also allow construction of broadly based protein interaction maps of rice.Graduate raining will be provided in areas that will combine genome sequence analysis with protein interaction analysis. Such skills will be crucial as the community grapples with integrating these disparate, large datasets. In addition, the approached developed could create a paradigm shift that could have a significant impact in defining a new strategy for analyzing complex plant genomes.The data resulting from the work will be available at:http://www.cshl.edu/genseq/riceproteome.