Integrated Transduction, Actuation, and Control for Cell-Based Sensing [UOM_FY05_059]

帕米拉，阿伯夏-0515873目标是开发和演示基于细胞的传感技术。细胞诊所是能够捕获和表征细胞的微环境。每一个“诊所”都是在一块芯片上制造的微型机电系统。生物系统具有高度的特异性、灵敏度和适应性，可以作为高度集成的传感器的一部分。第一个目标是利用反馈控制、集成电路和微流控工具进行样品制备、细胞加载和系统小型化。结果将被用于嗅觉传感和低假阳性病原体检测方面的两项持续努力。将从三个方面对该系统进行演示。(1)电渗流控制将从样品中去除所有光学可见(5微米)颗粒。这将清除污垢、灰尘和细菌，并留下气味，供嗅觉细胞传感器使用。该系统应具有足够高的吞吐量，以便实时使用。(2)用于在三维方向引导细胞的介电泳致动器将被用于在平面上定位细胞并将它们引导到细胞诊所小瓶中。(3)为了开发野战实用细胞传感器，将开发与细胞诊所相同维度的细胞导向视觉系统。拟议的技术进步将使基于细胞的传感走向实际实施并与真实样本一起使用。基于细胞的传感具有远远超过当今化学和生物传感器的选择性、灵敏度和速度的潜力。嗅觉和病原体检测问题直接关系到国家安全。这项技术在其他不同的领域也有明显的应用，如医疗保健、药物开发和环境监测。通过开发在微尺度上复杂操纵颗粒的基本技术和技术，集成的转换-驱动-控制方法预计将对基于细胞的传感之外的芯片实验室、微流体和纳米技术产生影响。PI是几个学科(流体和控制、微制造和共轭聚合物、集成电路和生物传感器)的工程师，与细胞生物学家、分子病理学家以及生物功能化表面和量子点方面的专家密切合作。PI正在率先开发可在无尘室外使用的MEMS教育套件。

ABSTRACTIIS-0515873Abshire, PamelaThe goal is to develop and demonstrate enabling technology for cell-basedsensing. Cell clinics are microenvironments that enable the capture and characterization of cells. Each "clinic" is a micro-electro-mechanical system fabricated on a CMOS chip. Biological systems have high specificity, sensitivity, and adaptability that can be part of a highly integrated sensor. The first goals are sample preparation, cell loading, and system miniaturization using the tools of feedback control, integrated circuits, and microfluidics. Results will leveraged into two ongoing efforts in olfactory sensing and low-false-positive pathogen detection. Three aspects of the system will be demonstrated. (1) Electroosmotic flow control will remove all optically visible (5 micron) particles from the sample. This will remove dirt, dust, and bacteria and leave behind odorants for presentation to the olfactory cell sensors. This system shall be capable of sufficiently high throughput to be used in real time. (2) Dielectrophoretic actuation for steering cells in three-dimensions will be used to position cells in the plane and to direct them into the cell clinic vials. (3)In order to develop field utility cell-based sensors, a vision system with the same dimensions as cell clinics for cell steering will be developed. The proposed technological advances will allow cell-based sensing to move toward actual implementation and use with real samples. Cell-based sensing has the potential for selectivity, sensitivity, and speed that far exceedtoday's chemical and biological sensors. Problems of olfactory sensing and pathogen detection are of immediate relevance to national security. This technology has clear applications in other diverse fields such as health care, pharmaceutical development, and environmental monitoring. The integrated transduction-actuation-control approach is expected to have an impact outside of cell-based sensing to labs-on-a-chip, microfluidics, and nanotechnology by developing basic technology and techniques for sophisticated manipulation of particles at the micro-scale. The PIs are engineers in several disciplines (fluids and controls, micro-fabrication and conjugated polymers, integrated circuits and biosensors) working closely with cell biologists, molecular pathologists, and experts in bio-functionalized surfaces and quantum dots. The PIs are pioneering the development of MEMS education kits that can be used outside of a clean room.