Arabidopsis 2010: Completing the Expression Catalog of the Arabidopsis Transcriptome by Quantitative Real Time PCR

几个大型项目已经生成了模式植物拟南芥基因组中27000多个基因中的23000个基因的表达数据。然而，其余4000多个基因的表达水平无法通过迄今使用的方法可靠地检测到，并且对这组基因的功能知之甚少。该项目的目的是为这些4000多个拟南芥基因开发一个在各种组织和条件下的定量表达谱目录，这些基因要么低于Affymetrix ATH1阵列可检测的水平，要么使用高通量实时定量PCR在阵列上没有表示，这种技术比以前的方法灵敏得多。通过该项目的结论，拟南芥和植物研究界将在各种实验条件下获得拟南芥基因组中几乎所有基因的基因表达谱，这是2010年项目目标的一个重要里程碑。将这4000多个低表达基因的表达模式与大数据集中已有的大约23000个更好表征的基因进行比较，将为这组知之甚少的基因的生物学功能提供新的见解。同时，在更小的范围内，利用敏感的报告基因结构确定约1000个这些基因的空间表达谱，从而进一步了解它们在植物中的作用。该项目的描述可在http://www.tigr.org/tdb/e2k1/ath1/qpcr/上获得；基因表达数据将在处理过程中公布在该网站以及两个社区数据库TAIR （www.arabidopsis.org）和NASC （www.arabidopsis.info）上。本项目开发的分子和生物资源将通过拟南芥生物库存中心（http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/abrchome.htm）进行分发。在这项工作的过程中，本科生和高中教师将被邀请到实验室，为前者提供宝贵的实践研究经验，为后者提供足够的材料熟悉，以便能够将他们的工作转化为新的课堂课程。

Several large-scale projects have generated expression data for approximately 23,000 of the 27,000+ genes in the genome of the model plant Arabidopsis thaliana. However, the remaining 4,000+ genes are expressed at levels that are not reliably detected by the methods used to date and very little is known about the functions of this set of genes. The aim of this project is to develop a catalog of quantitative expression profiles under a variety of tissues and conditions for these 4,000+ Arabidopsis genes that either fall below levels detectable by the Affymetrix ATH1 array or are not represented on the array using high throughput quantitative real-time PCR, a technique that is much more sensitive than previous methods. By the conclusion of this project, the Arabidopsis and plant research communities will have gene expression profiles for essentially every gene in the Arabidopsis genome under a wide variety of experimental conditions, a major milestone in the goals of the 2010 project. Comparison of the expression patterns of these 4000+ low-expressed with the approximately 23,000 better characterized genes already available in the larger data sets will provide new insights into the biological functions of this set of poorly understood genes. In parallel, and on a smaller scale, the spatial expression profiles will be determined for ~ 1,000 of these genes using sensitive reporter gene constructs, thus providing further insight into their roles in the plant. A description of the project is available at http://www.tigr.org/tdb/e2k1/ath1/qpcr/; the gene expression data will be posted on that web site as it is processed, as well as at two community databases, TAIR (www.arabidopsis.org) and NASC (www.arabidopsis.info). Molecular and biological resources developed in this project will be distributed through the Arabidopsis Biological Stock Center (http://www.biosci.ohio-state.edu/~plantbio/Facilities/abrc/abrchome.htm). During the course of this work, undergraduates and high school teachers will be hosted in the laboratory, to provide the former with valuable hands-on research experience and the latter with sufficient familiarity with the materials to be able to translate their work into new classroom programs for their students.