Arabidopsis 2010: Chemical Genomics of Networks Controlling Vesicular Trafficking in Plant Development

科学价值：该项目旨在证明，将创新和最先进的化学基因组学方法与基因组工具相结合，可用于阐明控制拟南芥生长和发育关键的囊泡运输的基因网络。 植物特有的基因网络，例如，那些控制植物细胞分裂或植物激素生长素极性运输的基因，将在这个试验项目中特别感兴趣。 化学基因组学方法的提出，因为这些网络已被证明是棘手的传统的遗传/基因组学方法。 化学基因组学是一个新兴的领域，其中采用高通量技术来鉴定生物活性小分子，这些小分子反过来又用于询问复杂的生物过程或其潜在的基因网络。 将实现以下科学目标：1）发现一组干扰细胞分裂控制的小分子，这需要囊泡运输，2）通过将化学基因组学方法与通过先前2010年项目开发的基因组学工具（例如，SIGNAL），以及3）开发一个基于网络的机制，通过该机制，本项目产生的工具、材料、化学品和知识可以容易地传播给公众（http：//bioinfo.ucr.edu/projects/PlantChemBase/search.php;用户名：visitor，密码：chemical）。 该项目的长期目标是补充和加强现有的功能基因组学/基因组学工具，以发现拟南芥基因的功能。更广泛的影响：该项目将通过证明化学基因组学在基因功能和调控网络研究中的有用性，对植物生物学产生广泛的影响。本项目开发的工具和材料，例如，将向公众提供基于高通量成像的化学筛选程序和新的遗传学和分子工具，包括突变体、转基因系、蛋白质表达载体和亚细胞标记。将建立一个综合数据库，用于化合物挖掘、筛选数据检索、表型和可下载的开放源码化合物分析工具，并通过一套集中的网络服务公布。该项目将整合年轻科学家的培训，目标是为他们提供跨学科化学基因组学研究方面的广泛综合经验。 未来的科学家将接受良好的培训，为独立的学术生涯做好准备。 将开展有针对性的外展工作，以激发女性和代表性不足的少数族裔学生对这一科学前沿的兴趣。这种培训将产生新一代的多元化科学家，他们可以组成21世纪世纪研究事业所需的互补团队。

SCIENTIFIC MERIT: This project is to demonstrate that integration of an innovative and state-of-the-art chemical genomics approach with genomic tools is useful for elucidating networks of genes that control vesicular trafficking critical for Arabidopsis growth and development. Gene networks that are specific to plants, e.g., those that control plant cell division or polar transport of the plant hormone auxin, will be of particular interest in this pilot project. The chemical genomics approach is proposed because these networks have proven intractable to traditional genetic/genomics approaches alone. Chemical genomics is an emerging field, in which high-throughput technologies are employed to identify bioactive small molecules, which in turn are used to interrogate a complex biological process or its underlying network of genes. The following scientific goals will be achieved: 1) Discover a battery of small molecules that perturb the control of cell division that requires vesicular trafficking, 2) Initiate assigning the functions to a network of genes controlling cell division in Arabidopsis by integrating the chemical genomics approach with the genomics tools developed through previous 2010 projects (e.g., SIGNAL), and 3) Develop a web-based mechanism by which tools, materials, chemicals, and knowledge generated in this project can be easily disseminated to the public (http://bioinfo.ucr.edu/projects/PlantChemBase/search.php; usename: visitor, password: chemical). The long-term goal of this project is to complement and enhance the existing functional genomics/genomics tools for the discovery of the functions of genes in Arabidopsis.BROADER IMPACT: This project will have a broad impact on plant biology by proving the usefulness of chemical genomics in the research on gene function and regulatory networks. Tools and materials, developed in this project, e.g., high-throughput imaging-based chemical screen routines and novel genetics and molecular tools including mutants, transgenic lines, protein expression vectors, and sub-cellular markers will be made available to the public. An integrated database for compound mining, retrieval of screening data, phenotypes and downloadable open-source tools for compound analysis will be created and published through a centralized suite of Web services. This project will integrate training of young scientists with the goal of providing them with a broad integrated experience within interdisciplinary chemical genomics research. The future scientists will be well trained and prepared for independent academic careers. Focused outreach efforts will be undertaken to stimulate interest in this leading edge of science among women and under-represented minority students. This training will produce a new generation of diverse scientists who can form complementary teams required for 21st century research careers.