SBIR Phase II: Portable Sequential Injection (SI)-High Performance Liquid Chromatography (HPLC) Analyzer

这个小型企业创新研究(SBIR)第二阶段研究项目旨在开发一种便携式和全自动的基于色谱的分析仪。该分析仪将集成几种成熟的技术，以生产一种紧凑、坚固、易于实施的自动化仪器，供在线、现场或现场使用，特别是在需要复杂的高效液相色谱(High Performance Liqumatography)分析的情况下。基于顺序进样(SI)方案和高效液相仪器，该设备将完全自动化，并提供关于样品采集、前处理、化学修饰、分离和目标分析物检测的集成方法。这种化学分析仪在开发过程中将利用几项新技术，包括顺序注射、便携式高压注射泵和溶胶-凝胶高效液相色谱柱。建议的SI-HPLC仪将在在线过程控制、现场环境监测或作为医疗、执法和军事用途的多用途现场就绪分析仪中得到应用。顺序进样技术使该分析仪非常适合未经培训的人员使用或远程自主分析，因为样品处理和制备可以完全自动化。SI-HPLC的最初目标用途将是在线生物过程控制(例如制药)，以便为质量控制或最佳产品产量提供实时反馈。

This Small Business Innovation Research (SBIR) Phase II research project is aimed at the development of a portable and fully automated chromatographic based analyzer. This analyzer will integrate several proven technologies to produce an automated instrument that is compact, robust and easily implemented for on-line, at-site, or field-ready use, especially where complex HPLC (High Performance Liquid Chromatography) analyses is needed. Based on both Sequential Injection (SI) protocol and HPLC instrumentation, this device will be fully automated and provide an integrated approach with respect to sample collection, pre-treatment, chemical modification, separation and detection of target analytes. This chemical analyzer will exploit several novel technologies including sequential injection, portable high-pressure syringe pumps and sol-gel HPLC columns in its development. The proposed SI-HPLC instrument will find applications in on-line process control, at-site environmental monitoring or as a multipurpose field-ready analyzer for medical, law-enforcement and military use. Sequential Injection technologies make this analyzer ideally suited for use by untrained personnel or for remote autonomous analysis since sample handling and preparation can be completely automated. Initial targeted use for the SI-HPLC will be for online bioprocess control (e.g. pharmaceuticals) to provide real-time feedback for Quality Control or optimal product yield.