SST: Collaborative Research: Integrated Optical and Electrical Single Molecule Sensors

0528730schmidtoptoptic和electrical sensing使得对气相或液相中单个分子的研究成为可能，从而在非常基础的水平上理解物理、化学和生物过程。提出了一种集成传感器平台，提供单分子分辨率，极小的气体或液体样品体积，平面结构和高通量的传感器阵列配置。这种传感器对于提供快速、可靠和强大的分析工具是非常理想的。不需要庞大的三维装置，这些传感器也可以在实验室之外使用，可以影响从基因组学和蛋白质组学到毒理学和空气和水的污染监测等各个领域。智力优势：提出的方法是基于纳米孔技术与单分子电分辨率和具有液体核的反谐振反射光学（ARROW）波导的结合。本研究的主要目标如下：1。构建一种新型集成光学传感器，同时提供单分子荧光灵敏度，并允许并行传感器阵列架构。2. 将纳米孔与光波导集成为“智能门”。纳米孔将控制光检测通道中的分子访问，同时提供单分子分辨率的电传感能力。利用光电传感的新组合来研究基因组学和蛋白质组学中的生物分子，以提高对分子生物学在单分子水平上的认识。虽然该概念原则上同样适用于气相分子的传感，但本提案的重点是用于单分子水平生物分子研究的液体集成传感器。更广泛的影响：上述研究计划具有显著的更广泛的影响。它将推进光学传感器科学和技术，并提供集成光学、分子生物学和半导体器件制造领域的研究生培训。本科研究将被纳入该计划。在加州大学圣迭戈分校，学生将在现有的UC LEADS（未被充分代表的少数民族）或COSMOS （K-12学生）项目下参与该项目。在杨百翰大学，本科生和来自弱势群体的高中生（杨百翰大学SOAR项目）将在夏季参与项目的制作。

0528730SchmidtOptical and electrical sensing has enabled the study of individual molecules in the gas or liquid phase, leading to an understanding of physical, chemical and biological processes on a very fundamental level. An integrated sensor platform is proposed that provides single-molecule resolution, extremely small gas or liquid sample volumes, planar architecture, and high throughput in a sensor array configuration. Such a sensor is highly desirable for providing fast, reliable and robust analytical tools. Without the need for bulky three-dimensional setup, these sensors can also be used outside a laboratory and could impact a variety of fields ranging from genomics and proteomics to toxicology and pollution monitoring in both air and water.Intellectual merit: The proposed approach is based on combining nanopore technology with single-molecule electrical resolution and antiresonant reflecting optical (ARROW) waveguides with liquid cores. The research has the following primary goals: 1. To build a new type of integrated optical sensor that simultaneously provides single-molecule fluorescence sensitivity and allows for parallel sensor array architectures. 2. To integrate nanopores as "smart gates" with the optical waveguides. The nanopores will control molecule access in the optical detection channel and simultaneously provide electrical sensing capability with single molecule resolution.3. To use the novel combination of optical and electrical sensing to study biomolecules in genomics and proteomics for enhancing understanding of molecular biology on the single molecule level. While the concept is, in principle, equally applicable to sensing of molecules in the gas phase, the focus of this proposal is on integrated sensors of liquids for biomolecular studies on the single molecule level.Broader Impact: The research program outlined above has significant broader impact. It will advance optical sensor science and technology and provide graduate student training in the fields of integrated optics, molecular biology, and semiconductor device fabrication. Undergraduate research will be integrated into the program. At UCSC, students will participate in the project under the existing UC LEADS (underrepresented minorities) or COSMOS (K-12 students) programs. At BYU, undergraduates as well as high school students from underrepresented groups (BYU SOAR program) will be involved in the fabrication aspects of the projects during the summer.