CAA: Identification of Genetic Networks in Neurospora Crassa: A Systems Biology Approach

本研究的主要目的是为生物体的代谢网络提供一个更清晰的视图。本文将重点研究奎宁酸在面包霉菌粗神经孢子虫体内的代谢。霉菌的代谢行为将在从蔗糖（一种有利的营养物质）到奎宁酸（一种不太理想的营养物质）的变化过程中进行检查。霉菌的代谢反应将通过使用分子和计算生物学方法测量生物体的信使核糖核酸水平来监测。总之，本研究产生的信息将为鉴定目前描述的代谢网络和揭示生物体中新的相互作用网络提供基础。关于有机体代谢网络的调节，还有许多未解之谜。此外，生物体内某一特定网络与整个代谢网络之间的联系还没有牢固建立。在这个项目中的方法将扩大在面包霉菌N. crassa代谢调节的知识，包括许多生物。这项研究揭示的信息将提供更多关于生物功能的知识，并为未来对生命代谢行为的研究奠定基础。该项目将为年轻科学家提供优秀的培训和指导，并帮助他们在生物学和其他科学学科领域做出职业选择，因此对代表性不足的学生的教育影响是显而易见的。

The primary objective of this research is to provide a clearer view of metabolic networks in living organisms. The research will be focused on the metabolism of quinic acid in the bread mold Neurospora crassa. The mold's metabolic behavior will be examined during a change from sucrose, which is a favorable nutrient, to quinic acid, a less desirable nutrient. The metabolic response of the mold will be monitored by measuring the organism's messenger ribonucleic acid levels by using molecular and computational biology methods. Altogether, the information produced in this research will provide the basis for the identification of currently described metabolic networks and reveal new interacting networks in living organisms.There are many unanswered questions about the regulation of an organism's metabolic network. In addition, the connection between a specific network and the entire metabolic network in living things is not firmly established. The approach in this project will expand the knowledge of metabolic regulation in the bread mold N. crassa to include many organisms. The information revealed in this research will provide more knowledge of what makes a living thing function and establish a foundation for future investigations on the metabolic behavior of life. The impact on the education of underrepresented students is clear as this project will provide excellent training and mentoring for young scientists and assist them in making career choices in the field of biology and other scientific disciplines.