Electrohydrodynamic Instability Micromixing Within Two Phase Microfluidic Systems for Biological Purification Technology

摘要提案编号：CTS-0553906主要研究者：Zahn，Jeffrey所属机构： 宾夕法尼亚州立大学提案标题：电流体动力学不稳定性微混合两相微流生物净化技术本项目旨在研究有机-水两相微流，并建立一个自主的装置，能够执行液-液萃取技术的生物样品净化技术。分析和计算方法。两相流行为将使用荧光染料定位技术和microPIV进行研究。的流动是不稳定的电流体动力学（EHD）的不稳定性，以提高两相的混合，以增加液体提取可能发生的表面积，监测一种新的侧视microPIV技术的三维轮廓重建。该提案将实验研究两相系统和EHD混合的流动行为，并将结果与分析和计算分析进行比较。EHD不稳定性的建模计算建模也将得到扩展。更广泛的影响这个问题是多学科的，汇集了工程和生物学的工具来解决一个问题，这是至关重要的任何未来的应用程序，涉及芯片上的样品制备。这项工作整合了跨学科技术，并研究了一种迄今为止很少或根本没有受到关注的DNA提取方法。该技术将使样品制备高度集成的遗传筛查诊断。控制两相混合是任何未来应用的关键障碍，该应用在分析之前使用液体萃取分离程序进行样品纯化。该技术有望在医学基因检测、高通量DNA测序、生物战剂检测和水质监测等不同领域实现现场生物样本收集和分析。将特别强调在大学科学教育中向代表性不足的群体进行教育宣传。这些活动将加强在新兴的BioMEMS和纳米生物技术领域的基础知识，以及培训该地区的年轻科学家，工程师和教育工作者。

ABSTRACTProposal Number: CTS-0553906Principal Investigator: Zahn, JeffreyAffiliation: Pennsylvania State UniversityProposal Title: Electrohydrodynamic Instability Micromixing Within Two Phase Microfluidic Systems for Biological Purification TechnologyThis project proposes to study organic-aqueous two phase microflows, and create an autonomous device capable of performing liquid-liquid extraction techniques for biological sample purification.Intellectual MeritThe work will extend the current understanding and manipulation of multiphase flows in microgeometries through experimental, analytical and computational approaches. Two phase flow behavior will be studied using a fluorescent dye localization technique and microPIV. The flow is destabilized by an electrohydrodynamic (EHD) instability to enhance mixing of the two phases to increase the surface area over which liquid extraction may occur, monitored by a novel sideview microPIV technique for three dimensional profile reconstruction. The proposal will experimentally study the flow behavior of the two phase system and EHD mixing and will compare the results to analytical and computational analysis. The modeling of EHD instabilities by computational modeling will also be extended.Broader ImpactThe problem is multidisciplinary, bringing together tools from engineering and biology to attack a problem that is crucial for any future applications involving on-chip sample preparations. This work integrates interdisciplinary technologies and investigates a powerful approach to DNA extraction that has received little or no attention to date. This technique will enable sample preparation for highly integrated genetic screening diagnostics. Controlling two-phase mixing is a key hurdle for any future application that uses a liquid extraction separation procedure for sample purification prior to analysis. This technique holds major promise to enabling on-site biological sample collection and analysis for areas as disparate as medical genetic testing, high throughput DNA sequencing, biowarfare agent detection, and water quality monitoring. Special emphasis will be placed on educational outreach to under-represented groups in college level science education. These activities will enhance fundamental knowledge in the emerging field of BioMEMS and nanobiotechnology as well as train young scientists, engineers and educators in the area.