Thermodynamic Modeling of Fluorescent In Situ Hybridization (FISH) for Environmental Applications

0606894 Noguera拟议的研究继续PI先前的研究，以基于生物学的模型来预测甲酰胺解离曲线，这是使用FISH方案的关键步骤，FISH方案是一种广泛用于微生物鉴定研究的分子生物学工具。这个概念以前被证明为E。大肠杆菌;下一步是证明相同的建模方法对其他五种不同的细菌/古细菌的适用性。如果这项工作成功，该项目可以显着提高设计FISH探针的效率，并产生广泛的有益影响。该提案包括一项计划，即创建一个互联网工具，使微生物生态学和环境工程领域的研究人员能够获得该项目的结果。

0606894NogueraThe proposed research continues the PI's previous research into a thermodynamics-based model to predict formamide dissociation curves, a key step in use of the FISH protocol, a molecular biology tool widely used in microbial identification studies. The concept was previously demonstrated for E. coli; the next step is to demonstrate the applicability of the same modeling approach to five other diverse bacteria/archea. If the work is successful, this project could significantly improve the efficiency of designing FISH probes and have widespread beneficial impacts. The proposal includes a plan to create an Internet tool to make results of the project available researchers in microbial ecology and environmental engineering.