Advancement of a Sustainable Microbial Wastewater Treatment Process for the Removal of Phosphorus

0607329Logs为防止地表水富营养化，许多排放标准正在接近0.01至0.02MGP L-1的值。强化生物除磷技术被广泛应用于污水的去除，出水浓度从1-5mgP L-1不等，需要进行化学处理才能达到更严格的标准。EBPR也经常被证明是不稳定和不可靠的。这项研究将为EBPR提出一个新的代谢模型，该模型提出该过程是由废水处理中不平衡的生长条件导致的微生物严格反应驱动的。通过最大限度地扩大严格响应的范围和持续时间，出水浓度可以降低到0.01mgP L-1到0.02mgP L-1。这项研究将通过四项不同的任务进行。在任务1下，我们将通过对实验室规模的生物反应器使用真实废水和野生微生物协会操作的时间序列蛋白质组数据进行多变量统计分析，确认与拟议的EBPR严格响应模型相关的关键代谢过程。任务2和任务3将确定生物废水处理中的最佳反应器运行参数，其中本土微生物联合体将完成生物除磷至微不足道的水平，同时完成所有其他生物处理目标。任务4将制定工艺扩大的设计和操作标准。对微生物严格反应的新理解将导致更可持续的废水处理，减少对化学品的需求，减少资本和运营成本，并防止生物固体处理的长期问题。

0607329LogeMany effluent standards are approaching values of 0.01 to 0.02 mgP L-1 to prevent eutrophication of surface waters. Enhanced biological phosphorus removal (EBPR) is used extensively with removals resulting in effluent concentrations ranging from 1-5 mgP L-1, requiring chemical treatment to meet stricter standards. EBPR also often proves to be unstable and unreliable. This study will advance a new metabolic model for EBPR that proposes the process is driven by the microbial stringent response that results from unbalanced growth conditions within wastewater treatment. By maximizing the extent and duration of the stringent response, effluent concentrations can be reduced to 0.01 to 0.02 mgP L-1. The study will be undertaken through four discrete tasks. Under Task 1 we will confirm the critical metabolic processes associated with the proposed EBPR-Stringent Response model through multivariate statistical analyses of time-series proteomics data derived from laboratory-scale bioreactors operated with real wastewater and wild microbial consortia. Tasks 2 and 3 will establish the optimum reactor operational parameters within biological wastewater treatment wherein the indigenous microbial consortium will accomplish biological phosphorus removal to negligible levels while concurrently accomplishing all other biological treatment objectives. Task 4 will develop design and operational criteria for process scale-up. The new understanding of the microbial stringent response will result in more sustainable wastewater treatment with decreased demand for chemicals, less capital and operations costs, and prevention of long-term problems with biosolids treatment.