MRI: Development of a 4D micro/nano-scale defocused particle tracking (DPT) imaging technique and its applications in nanobiotechnology

建议没有。cts -0619626首席研究员：Abraham D. Stroock，康奈尔大学4维微/纳米尺度散焦粒子跟踪（DPT）成像技术的发展及其在纳米生物技术中的应用这项主要研究仪器拨款旨在开发一种成像技术，通过定量使用荧光显微镜拍摄的散焦图像，以亚微米的空间和毫秒级的时间分辨率跟踪多个微/纳米尺度的粒子。这项新技术被称为四维微/纳米散焦粒子跟踪（4D DPT）技术。4D DPT技术对当前最先进的技术的主要贡献是它能够在微纳米尺度上探测粒子的拉格朗日信息，这对于微/纳米流体器件、bioMEMS器件的发展以及生物学中细胞间和细胞内动力学的理解至关重要。4D微纳DPT技术代表了微纳尺度流动可视化的重要创新。拟议项目的智力价值在于它能够以前所未有的空间和时间分辨率揭示微/纳米流体和生物流体系统的动态行为。结合生物光子学的最新进展，特别是基因编码荧光试剂，4D微/纳米DPT技术将能够可视化活细胞中的单分子事件。这种能力，反过来，将发挥独特的作用，在最近的探索相关的基因网络动力学与细胞的表型生物学。就更广泛的影响而言，4D DPT将作为康奈尔纳米生物技术中心（NBTC - NSF STC）的共享设施，它将为许多研究学科的用户之间的合作提供一个自然的环境。因此，它将影响一个广泛的科学界。此外，4D DPT技术将通过NBTC的工业合作计划以及pi与当地工业的合作提供给工业。由于实验结果的视觉性，4D成像系统为我们提供了一个独特的机会来接触公众。4D DPT成像系统的结果将在伊萨卡科学中心以“布朗运动”为主题进行展示，参观者可以看到稀释的纳米和微粒子的电影片段，以及游动的细菌。pi将为K-12社区和研究界开发一个互动网站。为了让更多的研究界人士了解最新的研究成果和影像软件，各研究项目将建立和维护一个最新的网站。

Proposal No. CTS-0619626Principal Investigator: Abraham D. Stroock, Cornell UniversityMRI: Development of a 4-dimensional micro/nano-scale defocused particle tracking (DPT) imaging technique and its applications in nanobiotechnology This Major Research Instrumentation grant is to develop an imaging technology for tracking multiple micro/nano scale particles with sub-micrometer spatial and millisecond temporal resolution via a quantitative use of defocused images taken with an epifluorescence microscope. This new technology is referred to as a 4-dimensional micro/nano defocused particle tracking (4D DPT) technique. The main contribution of the 4D DPT technique to the current state-of-art technology is its capability to probe Lagrangian information of particles at micro and nano scales, which is essential for the development of micro/nano fluidic devices, bioMEMS device, as well as the understanding of inter and intra cellular dynamics in biology. The 4D micro/nano DPT technique represents an important innovation in flow visualization at micro/nano meter scales. The intellectual merit of the proposed project resides in its ability to unravel dynamic behavior both in micro/nano fluidic and biofluidic systems at unprecedented spatial and temporal resolution. When coupled with recent advances in biophotonics, particularly genetically encoded fluorescent reagents, 4D micro/nano DPT technique will enable an ability to visualize single molecular events in living cells. Such capability will, in turn, play a unique role in the recent quest of correlating gene network dynamics with cell's phenotype in biology. In terms of broader impact, the 4D DPT will serve as a shared facility within the Cornell Nanobiotechnology Center (NBTC - NSF STC), it will provide a natural setting for collaboration among its users across many research disciplines. As such, it will impact a broad scientific community. In addition, the 4D DPT technology will be made available to industry through the NBTC's industrial partnership program, and through PIs' collaboration with local industries. The 4D imaging system provides us with a unique opportunity to reach out to the public due to the visual nature of the experimental results. The results of the 4D DPT imaging system will be made into an exhibition entitled `Brownian motion' at the Ithaca Science Center, where visitors can see movie clips of dilute nano and micro particles, as well as swimming bacteria. The PIs will develop an interactive website both for the K-12 community and the research community. For the greater research community, the PIs will establish and maintain an up-to-date website of research results and imaging software.