Systems Approach to Dynamic Atomic Force Microscopy

摘要：本文旨在建立调幅原子力显微镜（AFM）中基于模型的同时形貌和参数估计框架。将开发适合于实时识别的尖端-样品相互作用的参数模型。谐波平衡和功率平衡工具将被纳入模型参数的估计。基于该模型的振幅和相位动力学将被开发，这将用于用实验数据验证模型，随后用于控制设计目的。这些方法将用于研究酵母细胞。本文将研究一种尖端-样本相互作用势的非参数重构框架。限制如何很好地调幅AFM可以破译不同的样品相互作用将被研究。PI将继续通过交换学生访问和举办专门讲习班，推动物理和工程社区之间的技术转让。将开发一种基于网络的原子力显微镜远程操作系统，并将其集成到纳米审讯课程中。微悬臂在不同领域的应用影响越来越大，对科学产生了根本性的影响。事实上，它们为纳米技术的发展提供了重要的推动力。本文研究的调幅原子力显微镜方法是目前使用最多的原子力显微镜方法，因为它可以以原子分辨率对样品进行成像，并且是非侵入性的，因此对生物技术相关的研究特别有用。提出的方法是可行的，因此将为研究基础科学打开大门。PI与领先的生物相关扫描探针显微镜公司有着密切的合作关系。这项合作有望促进PI学术机构和SPM行业之间在该计划中开发的理论和技术的转移。根据本建议开发的基于网络的afm远程操作将有助于激发人们对科学的兴趣，并将形成一种教学工具。

ABSTRACT:The goal of this proposal is to establish a framework for model based simultaneous topography and parameter estimation in the amplitude modulation atomic force microscopy (AFM). Parametric models of tip-sample interaction that are amenable to real-time identification will be developed. Harmonic balance and power balance tools will be incorporated towards the estimation of the model parameters. The amplitude and phase dynamics based on the model will be developed, which will be used to validate the model with experimental data and subsequently used for control design purposes. These methods will be used to study yeast cells. A framework for non-parametric reconstruction of tip-sample interaction potential will be researched. Limitations on how well amplitude modulated AFM can decipher different sample interactions will be studied. The PI will continue to push the synergistic transfer of know-how between the physics and engineering communities by exchanging student visits and presenting specialized workshops. A web based remote operation of the AFM will be developed and integrated into a course on nano-interrogation.Micro-cantilevers are being used in diverse areas with increasing impact and have influenced science in a fundamental manner. Indeed, they provided a significant impetus to nanotechnology. The amplitude modulation atomic force microscopy method to be researched in this proposal is by far is the most used AFM mode as it can image samples with atomic resolution and is non-invasive and is thus particularly useful for biotechnology related investigation. The methods proposed are enabling and therefore will open doors for investigating basic science. .The PI has strong collaboration with leading biology related scanning probe microscope companies. This collaboration is expected to foster transfer of the theory and technology developed in this program between the academic institution of the PI and the SPM industry. The web-based remote operation of AFMs to be developed under this proposal will help excite interest in science and will form a instructional tool.