SGER: Exploration and Quantification of Ion Gradients in a Capillary Microdevice

建议编号：CTS-0636254主要研究员：Minerick，Adrienne R.从属关系：密西西比州立大学建议标题：SGER：探索和量化毛细微设备中的离子梯度智力优点：开发易于筛选pH梯度的微设备的工具将引起学术界和工业界的实际兴趣。这些信息，再加上对选择性离子运动及其对壁电荷的动态影响的日益深入的理解，是学术和工业微器件研究人员的智力兴趣。等电点测定的初步结果表明，在含有电极的2毫升样品井中，pH值发生了显著变化。下一步是直接测量微设备通道内的任何pH梯度等高线。直型熔融石英毛细管微设备是进一步了解离子如何沿毛细管长度有选择地沿轴向移动以及径向朝向或远离毛细管壁移动的理想系统。这项工作将把直接测量数据与理论预测进行比较，并在必要时对模型进行修改。该项目将促进研究生和本科生的智力发展。广泛的影响：拟议的研究将能够确定微器件内的pH值和其他离子梯度，并开发一种用于筛选各种应用的微器件的工具，产生广泛的影响。这项技术还可以将等电聚焦技术带到纳米升液体的规模。在这个为期一年的项目期间，研究生和本科生的教育和专业发展将继续下去。成功的研究和教育技术将广泛传播给学术界和微技术界。

ABSTRACTProposal Number: CTS-0636254Principal Investigator: Minerick, Adrienne R.Affiliation: Mississippi State UniversityProposal Title: SGER: Exploration and Quantification of Ion Gradients in a Capillary Microdevice Intellectual Merit: The development of tools to easily screen microdevices for pH gradients would be of practical interest to academic and industrial communities. This information coupled with a growing understanding of selective ion movement and its dynamic impact on wall charge is of intellectual interest to academic and industrial microdevice researchers. Preliminary results by the PI show pH changes significantly in 2 mL sample wells containing electrodes. The next step is to directly measure any pH gradient contours within the channels of the microdevice. A straight, fused silica capillary microdevice is an ideal system with which to further understand how ions selectively move axially down the length of the capillary as well as radially either toward or away from the capillary walls. This work will compare direct measurement data to theoretical predictions and develop modifications to models if necessary. This project will promote the intellectual development of a graduate and an undergraduate student.Broader Impacts: The proposed research would enable determination of pH and other ion gradients within microdevices with the broad impact of developing a tool for the screening of microdevices for various applications. This technology could also bring isoelectric focusing techniques to the scale of nanoliters of fluid. The educational and professional development of graduate and undergraduate students will continue during the duration of this one-year project. Successful research and educational techniques will be broadly disseminated to the academic, and microtechnological communities.