SGER: An Autonomous Microbial Genosensor for Freshwater Microbial Ecology

该奖项将解决淡水细菌群落研究中最重要的挑战之一；具有以高频率和高度特异性远程采样和检测细菌的能力。将构建一个具有样本采集、过滤、细胞裂解、RNA提取/纯化/浓缩、基因扩增和数据传输能力的原位传感系统原型。该系统将设计为在湖泊中进行多天部署，并配备有检测两种常见淡水细菌的能力。该奖项将通过本科生、研究生和少数族裔培训产生重大而广泛的影响。

ABSTRACTThis award will address one of the most significant challenges in studying freshwater bacterial communities; having the ability to remotely sample and detect bacteria at high frequencies and with a high degree of specificity. A prototype insitu sensing system will be constructed with sample collection, filtration, cell lysis, RNA extraction/purification/concentration gene amplification, and data transmission capabilities onboard. The system will be designed for multi-day deployment in lakes and equipped with the ability to detect two common classes of freshwater bacteria. The award will have significant broader impacts through undergraduate, graduate, and minority training.