CAREER: Voltammetric Ion-Selective Electrode for Biological Polyions and Beyond

匹兹堡大学的Shigeru Ameiya教授获得了分析和表面化学计划颁发的职业奖，以开发用于生物多离子和其他氧化还原非活性离子的伏安离子选择电极(ISES)，这些离子在生物和环境介质中普遍存在且非常重要。基于离子选择聚合物和共轭导电聚合物的双聚合物膜将在固体电极上形成。电流响应将由离子选择膜和水样溶液之间的界面上的离子转移控制。导电聚合物将作为中间层，有效地调节离子导电膜和电子导电固体载体之间的电荷传输。离子电流响应将是监测生物医学上重要的多离子，如抗凝剂/抗血栓肝素及其解毒剂鱼精蛋白在其狭窄和低治疗浓度范围内的理想选择。本课程将探讨这些固载伏安离子选择电极的选择性、检出限和生物相容性的化学基础，以及微流控器件的小型化和并入策略。PI将通过将研究成果直接落实到教学课程中，将研究和教学计划整合在一起。伏安法ISES也将被开发为一个共享的教育工具，以解决本科实验室中出现的高氯酸盐污染的环境问题。PI将引入COMSOL多物理作为一种强大的模拟工具，以提高学生理解化学中计算密集型问题的能力。从研究中获得的信息将增强我们在化学传感器、电化学、分子识别和聚合物化学领域的知识和理解。伏安离子选择电极在生物医学、生物和环境分析中具有广泛的应用潜力。PI将利用跨学科的方法，扩大本科生、女性和代表性不足的少数族裔的研究参与。

Professor Shigeru Amemiya of the University of Pittsburgh has received a CAREER Award from the Analytical and Surface Chemistry Program to develop voltammetric ion-selective electrodes (ISEs) for biological polyions and other redox-inactive ions, which are ubiquitous and important in biological and environmental media. Double polymer membranes based on an ion-selective polymer and a conjugated conducting polymer will be created on a solid electrode. A current response will be controlled by ion transfer at the interface between the ion-selective membrane and the aqueous sample solution. The conducting polymer will serve as an intermediate layer to efficiently mediate charge transport between the ionically conductive membrane and the electronically conductive solid support. The ionic current response will be idealistic for monitoring biomedically important polyions such as anticoagulant/antithrombotic heparin and its antidote protamine in their narrow and low therapeutic concentration ranges. The chemical basis of selectivity, detection limit, and biocompatibility in these solid-supported voltammetric ISEs will be explored, as well as strategies for miniaturization and incorporation into microfluidic devices. The PI will integrate research and teaching programs by directly implementing research outcomes into teaching curricula. Voltammetric ISEs will be developed also as a shared educational tool to address the emerging environmental problem of perchlorate contamination in undergraduate laboratory. The PI will introduce COMSOL Multiphysics as a powerful simulation tool to enhance students' ability to understand computationally intense problems in chemistry.Information obtained from the research will enhance our knowledge and understanding in the areas of chemical sensors, electrochemistry, molecular recognition, and polymer chemistry. The voltammetric ISEs have potential for wide use in biomedical, biological, and environmental analyses. The PI will take advantage of the interdisciplinary approach to widen research participation of undergraduates, women, and underrepresented minorities.