Voltammetric Analysis of Female Reproduction

女性生殖的伏安分析

• 批准号: 1256799
• 负责人: Robert Meisel
• 金额: $ 60万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-15 至 2018-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1256799&HistoricalAwards=false
• 关键词: Voltammetric; Analysis; Female; Reproduction

A critical component of successful reproduction in female mammals involves integration with the male's reproductive state. How the female performs this integration is understood at the behavioral level, but is still poorly understood at the neurobiological level. A neural model will be developed to precisely measure chemical signaling of dopamine in a particular brain region (the nucleus accumbens) where dopaminergic activity is temporally synchronized with behavioral interactions with the male for successful reproduction. A technically sophisticated and innovative approach know as fast-scan cyclic voltammetry, combined with electrophysiological recording, will be used to measure dopamine release in the female's nucleus accumbens during reproduction and determine how nerve cells respond to dopamine-mediated signals. The fast scan cyclic voltammetry has the capability of measuring dynamic changes in dopamine signaling by taking measurements every 100 milliseconds, thus temporally coupling dopamine signaling and behavioral responses. This project will not only identify how the nervous system solves a complex problem of reproductive integration of brain and behavioral activities, but will also open up new avenues for detailed analyses of the neural regulation of reproduction and other social behaviors. The transfer of technology from Dr. Paul Garris's lab (Dr. Garris is a world leader in the development of fast-scan cyclic voltammetry) at Illinois State University to the University of Minnesota is a significant impact of the research goals. The project will also provide a platform for making this technology available to a range of investigators throughout the country. The development of this technology and subsequent dissemination will include making the raw data available online as well as creating online simulations that can be used as instructional tools through OmniScience (www.omnisci.org). The project will provide an important research base for the inclusion of undergraduate students, particularly underrepresented minority students, as a component of their science education and literacy.

雌性哺乳动物成功繁殖的一个关键组成部分涉及与雄性生殖状态的整合。 女性如何进行这种整合在行为层面上已得到理解，但在神经生物学层面上仍然知之甚少。 将开发一种神经模型，以精确测量特定脑区（中脑核）中多巴胺的化学信号，在该脑区中，多巴胺能活动与成功繁殖的雄性行为相互作用在时间上同步。 一种被称为快速扫描循环伏安法的技术复杂和创新的方法，与电生理记录相结合，将被用来测量生殖过程中女性丘脑核中多巴胺的释放，并确定神经细胞如何对多巴胺介导的信号做出反应。 快速扫描循环伏安法具有通过每100毫秒进行测量来测量多巴胺信号传导中的动态变化的能力，从而在时间上耦合多巴胺信号传导和行为反应。该项目不仅将确定神经系统如何解决大脑和行为活动的生殖整合的复杂问题，而且还将为详细分析生殖和其他社会行为的神经调节开辟新的途径。 从伊利诺斯州州立大学的Paul Garris博士实验室（Garris博士是快速扫描循环伏安法开发的世界领导者）到明尼苏达大学的技术转让对研究目标产生了重大影响。该项目还将提供一个平台，使全国各地的一系列调查人员都能使用这项技术。 这项技术的开发和随后的传播将包括在线提供原始数据，以及通过OmniScience（www.omnisci.org）创建可用作教学工具的在线模拟。 该项目将提供一个重要的研究基础，将本科生，特别是代表性不足的少数民族学生纳入科学教育和扫盲。