Ionome to the Genome: Mapping the Gene Networks Controlling Nutrient Content in Rice Grain

PI：大卫盐（普渡大学）CoPI：玛丽卢Guerinot（达特茅斯大学; subawardee），和香农平森（得克萨斯A M大学; subawardee）高级人员：李Tarpley（得克萨斯A M大学）和张敏（普渡大学）矿物质营养素，如钙，铁和锌在人类健康中发挥重要作用。开发稻米品种，使谷物中的矿物质营养成分增加，各种有毒微量矿物质含量降低，可对人类健康产生重大的积极影响，因为解决这种隐性饥饿的粮食解决方案为这些营养不良问题提供了可持续的解决方案。 该项目将利用在模型系统中所做的工作，并使用高通量元素分析，基因组学，水稻育种和生物信息学来揭示调节水稻谷粒矿物质组成的基因和基因网络。 作为一个起点，该项目将利用和探索世界各地种植的数千种水稻品种中米粒矿物质含量的自然变化。 水稻谷粒离子组学数据、DNA微阵列基因分型、转录物谱和作图数据以及产生的QTL信息将通过基于网络的普渡离子组学信息管理系统（PiiMS）（www.purdue.edu/dp/ionomics/）、Gramene（http：//www.gramene.org/）和基因表达综合数据库（GEO）（http：//www.ncbi.nlm.nih.gov/geo/）提供。该项目的更广泛影响将包括促进增加植物中的生物可利用矿物质营养素，并解决人类健康方面的重要问题。如果成功的话，营养丰富的种子也会带来农学上的好处，如增加幼苗活力，抵抗疾病和其他压力，增加作物产量。此外，了解有毒金属在植物中积累的途径将使作物的发展能够排除有毒金属并创造更健康的食物来源，或从土壤中提取有毒金属作为清理污染土地和水的战略。教育推广将包括在生态学领域培训博士后研究员、本科生和研究生。公众外联工作还将包括通过目前正在筹备的基因组学展览（www.genomicsexplorer.com）向科学博物馆提供捐助。

PI: David Salt (Purdue University)CoPIs: Mary Lou Guerinot (Dartmouth University; subawardee), and Shannon Pinson (Texas A&M University; subawardee)Senior Personnel: Lee Tarpley (Texas A&M University) and Min Zhang (Purdue University)Mineral nutrients such as calcium, iron and zinc play important roles in human health. The development of rice varieties that produce grain with increased mineral nutrient contents and decreased levels of various toxic trace minerals could have a significant positive impact on human health, as food-based solutions to this hidden hunger offer sustainable solutions to these problems of malnutrition. This project will leverage work done in model systems and use high throughput elemental analysis, genomics, rice breeding and bioinformatics to uncover the genes and gene networks that regulate the mineral composition of rice grain. As a starting point, the project will take advantage of and explore the natural variation that exists in rice grain mineral content in the many thousands of rice varieties grown throughout the world. The rice grain ionomic data, DNA microarray genotyping, transcript profiling and mapping data, and QTL information generated will be made available via the web-based Purdue Ionomics Information Management System (PiiMS) (www.purdue.edu/dp/ionomics/), Gramene (http://www.gramene.org/) and Gene Expression Omnibus (GEO) (http://www.ncbi.nlm.nih.gov/geo/). The broader impacts of this project will include contributing to increasing bio-available mineral nutrients in plants, and to solving important problems in human health. If successful, nutrient-rich seed should also lead to agronomic benefits such as increased seedling vigor, resistance to disease and other stresses, and increased crop yields. Furthermore, understanding the pathways by which toxic metals accumulate in plants will enable the development of crops to exclude toxic metals and create healthier food sources, or to extract toxic metals from the soil as a strategy to clean up polluted lands and water. Educational outreach will include training of postdoctoral fellows, undergraduates and graduate students in the field of ionomics. Public outreach efforts will also include contributions to science museums through a genomics exhibit which is currently under development (www.genomicsexplorer.com).