Empowering research with ultra-fast high-throughput genome sequencing on the benchtop

通过台式超快速高通量基因组测序增强研究能力

• 批准号: BB/M012360/1
• 负责人: David Salt
• 金额: $ 42.53万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM012360%2F1
• 关键词: Empowering; research; ultra; fast; throughput

Biological research is in the midst of a revolutionary change. This revolution is being driven by the explosive increase in our ability to rapidly and cheaply sequence whole genomes which has gone from taking 10 years to sequence a human genome to just 1 day. Such an information explosion is spurring massive advances in biological research across many disciplines, and holds tremendous promise for surmounting many of the major challenges facing the UK and the world, including improved human health, sustainable food production, protection of the environment, and renewable bioenergy. From its inception such NGS technology has generally been provided as a service to researchers by large-scale sequencing 'factories' with the financial capacity to invest in the expensive capital equipment required. Though effective, such service providers tend to lack flexibility and responsiveness, raising barriers to the productive uptake of NGS in both smaller laboratories and in fields not traditionally associated with genomics. This has in many ways led to a slower uptake of NGS in many fields of high priority to the BBSRC such as food security, bioenergy and environmental change. The University of Aberdeen is particularly strong in BBSRC funded research relevant to these priority areas, including research into fish physiology, crop genetics, plant and soil interactions, tree pathology, and tick and mite biology. Unfortunately, these communities are under served in this current climate of large-scale NGS providers. Acquisition of the new Illumina NextSeq 500 DNA sequencer within the University of Aberdeen's Centre for Genome-Enabled Biology and Medicine (CGEBM) will help to overcome this barrier to use, leading to an enhanced utilisation of genomics within these currently underserved BBSRC funded research communities at the University of Aberdeen and locally. The new capacity provided by the NextSeq 500 will also enable Aberdeen's continued excellence in research relating to human health, including healthy ageing and infection biology; areas where researchers at the University of Aberdeen have been exploiting high through put genomics for over a decade. Further, it is our experience that initiation of new applications of NGS that make real advances in understanding, often requires developmental work, which is more easily achieved through direct interaction with those running the facilities, a process made much easier if these facilities are local.

生物学研究正处于革命性的变革之中。这场革命是由我们快速、廉价地对整个基因组进行测序的能力的爆炸式增长所推动的，对人类基因组进行测序的时间从需要 10 年缩短到只需 1 天。这种信息爆炸正在推动许多学科的生物研究取得巨大进步，并为克服英国和世界面临的许多重大挑战带来了巨大希望，包括改善人类健康、可持续粮食生产、保护环境和可再生生物能源。从一开始，这种NGS技术通常是由大型测序“工厂”作为一项服务向研究人员提供，这些工厂有财力投资所需的昂贵资本设备。虽然有效，但此类服务提供商往往缺乏灵活性和响应能力，从而为小型实验室和传统上与基因组学无关的领域有效采用 NGS 带来了障碍。这在很多方面导致 BBSRC 在食品安全、生物能源和环境变化等许多 BBSRC 高度重视的领域中采用 NGS 的速度较慢。阿伯丁大学在 BBSRC 资助的与这些优先领域相关的研究方面尤其强大，包括鱼类生理学、作物遗传学、植物和土壤相互作用、树木病理学以及蜱和螨生物学的研究。不幸的是，在当前大规模 NGS 提供商的环境下，这些社区得不到服务。在阿伯丁大学基因组生物学和医学中心 (CGEBM) 内购买新型 Illumina NextSeq 500 DNA 测序仪将有助于克服这一使用障碍，从而在阿伯丁大学和当地目前服务不足的 BBSRC 资助的研究社区中提高基因组学的利用率。 NextSeq 500 提供的新能力也将使 Aberdeen 在人类健康相关研究领域（包括健康老龄化和感染生物学）持续取得卓越成就；阿伯丁大学的研究人员十多年来一直在这些领域开发高通量基因组学。此外，根据我们的经验，启动NGS新应用以在理解方面取得真正的进展，通常需要开发工作，而通过与设施运行人员的直接互动更容易实现这一点，如果这些设施是本地的，这一过程就会容易得多。

项目成果

Isolation of 'Candidatus Nitrosocosmicus franklandus', a novel ureolytic soil archaeal ammonia oxidiser with tolerance to high ammonia concentration.

• DOI: 10.1093/femsec/fiw057
• 发表时间: 2016-05
• 期刊: FEMS microbiology ecology
• 影响因子: 4.2
• 作者: Lehtovirta-Morley LE;Ross J;Hink L;Weber EB;Gubry-Rangin C;Thion C;Prosser JI;Nicol GW
• 通讯作者: Nicol GW

The effects of graded levels of calorie restriction: IV. Non-linear change in behavioural phenotype of mice in response to short-term calorie restriction.

• DOI: 10.1038/srep13198
• 发表时间: 2015-08-25
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Lusseau D;Mitchell SE;Barros C;Derous D;Green C;Chen L;Han JD;Wang Y;Promislow DE;Douglas A;Speakman JR
• 通讯作者: Speakman JR

Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.).

• DOI: 10.1016/j.aquaculture.2016.07.017
• 发表时间: 2017-01-20
• 期刊: Aquaculture (Amsterdam, Netherlands)
• 作者: Dehler CE;Secombes CJ;Martin SA
• 通讯作者: Martin SA

Hsf1 and Hsp90 orchestrate temperature-dependent global transcriptional remodelling and chromatin architecture in Candida albicans.

• DOI: 10.1038/ncomms11704
• 发表时间: 2016-05-26
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Leach MD;Farrer RA;Tan K;Miao Z;Walker LA;Cuomo CA;Wheeler RT;Brown AJ;Wong KH;Cowen LE
• 通讯作者: Cowen LE

Lactate signalling regulates fungal ß-glucan masking and immune evasion.

乳酸信号调节真菌α-葡聚糖掩蔽和免疫逃避。

• 发表时间: 2016
• 作者: []