SBIR Phase I:Manufacturing Platform for Immunoassays

这项小型企业创新研究(SBIR)第一阶段研究使用电喷雾电离作为沉积方法，以快速且可重复地生成先进的生物传感器衬底。基于免疫分析的传感元件的生物活性表面沉积通常基于湿化学方法。不幸的是，在这种湿化学方法中，制备和共价连接步骤可能需要几天时间，并且由于复杂的多步骤过程而容易出现不一致。电喷雾电离提供了一种通过直接喷射沉积来解决这一问题的新方法。电离过程实现了直接光束聚焦，自然地导致了图案化和基于阵列的传感元件。电喷雾沉积不仅保证了对检测质量的更好控制，而且还使生产方案，如连续卷到卷加工成为可能。由于该工艺利用的是定向沉积，而不是来自液体覆盖层的扩散动力学，因此工艺时间为几分钟是可能的。使用电喷雾沉积免疫分析底物检测致病性大肠杆菌O157：H7细菌的初步结果表明，其灵敏度与湿化学制备的样品类似，并将进行进一步的优化。这项研究将改进生物传感器的生产方法，以更低的成本提供更高的质量，以提高生物传感器对该国的整体利用率。

This Small Business Innovation Research (SBIR) Phase I research uses electrospray ionization as a deposition method for rapid and reproducible generation of advanced biosensor substrates. Deposition of bioactive surfaces for immunoassay based sensing elements is usually based on a wet-chemical approach. Unfortunately, the preparation and covalent linkage steps in such wet-chemical approaches can take several days, and are prone to inconsistency due to the elaborate multi-step processes. Electrospray ionization offers the opportunity for a new approach to this problem by direct spray deposition. The ionization process enables direct beam focusing, leading naturally to patterning and array based sensing elements. Electrospray deposition not only promises better control over the assay quality, but also enables production schemes such as continuous roll-to-roll processing. Since the process leverages directed deposition rather than diffusion kinetics from a liquid overlayer, process times of minutes are possible. Preliminary results for detection of pathogenic E. coli O157:H7 bacteria using electrospray deposited immunoassay substrates have demonstrated sensitivities similar to wet chemically prepared samples, and further optimization will be performed. This research will lead to improved methods for production of biosensors that delivers higher quality at a reduced cost to improve the overall utility of biosensors to the country.