Identification of a New Component of the Photorespiratory C2 Cycle in Arabidopsis and Analysis of its Function in Metabolism

在我们的拟南芥EMS突变体中，参与光呼吸C2循环的蛋白质缺陷表明存在该途径的其他尚未确定的成分。我们的项目侧重于其中一个候选基因GLD1，其突变导致光呼吸条件下空气敏感表型和严重的甘氨酸积累（Somerville和Ogren， 1982）。我们已经将受影响的基因定位在2号染色体的左臂上，该基因不编码任何已知的参与C2循环的酶。我们想要鉴定基因，然后分析各自蛋白质的特性和代谢功能。这项工作将有助于更全面地了解植物主要代谢途径之一，即与光合作用、氮代谢和单碳代谢有关的代谢途径。

A re-examination in our group of Arabidopsis EMS mutants defective in proteins contributing to the photorespiratory C2 cycle indicates the presence of additional and as yet unidentified components of this pathway. Our project focuses on one of the candidate genes, GLD1, whose mutation leads to an air-sensitive phenotype and severe glycine accumulation under photorespiratory conditions (Somerville and Ogren, 1982). We have mapped the affected gene on the left arm of chromosome 2 which does not encode any enzyme known to contribute to the C2 cycle. We want to identify the gene to then analyse the properties and metabolic function of the respective protein. This work will contribute to a more complete knowledge of one of the major pathways of plant primary metabolism that is linked to photosynthesis, nitrogen metabolism, and one-carbon metabolism.