MRI: Acquisition of Instuments to Facilitate Molecular-Level Studies in Earth and Ocean Sciences

该提案要求为气相色谱仪飞行时间质谱仪和液相色谱仪-线性离子阱质谱仪提供资金，该质谱仪具有一个预备馏分收集器和一个多种样品电离模式和一个分析馏分收集器。该仪器将被PI和她的学生用于化学、生物和地质研究工作中复杂环境样品的表征和纯化生物分子。该设施将用于支持SIO现有的和拟议的研究工作，包括鉴定与深海细菌的温度和压力耐受性相关的膜脂和蛋白质修饰，以及膜脂生物合成的基因组学研究。该设施还将用于开发新的分析方法，并培训仪器操作和使用的研究生。外部用户需经邀请方可使用该设施。更广泛的影响PI在通过接待女高中生来促进女性参与科学方面有着良好的记录。拟议的仪器将补充和扩展SIO研究人员以及邀请的外部研究人员的能力。将为海洋化学和地球化学或海洋生物学系列研讨会举办研讨会，向更多的SIO民众展示设施的能力和成果

The proposal requests funds for a gas-chromatograph time-of-flight mass spectrometer with a preparatory fraction collector and a liquid chromatograph-linear ion trap mass spectrometer with multiple sample ionization modes and an analytical fraction collector. The instrumentation will be used by the PI and her students to characterize and purify biomolecules from complex environmental samples for chemical, biological and geological research efforts. The facility will be used to support existing and proposed research efforts at SIO, including identification of membrane lipid and protein modifications associated with temperature and pressure tolerance of deep-ocean bacteria, and genomics enabled studies of membrane lipid biosynthesis. The facility will also be used to develop new analytical methods, and train graduate students in instrumentation operation and use. Use of the facility by outside users is by invitation.Broader impactsThe PI has a strong record of enhancing the participation of women in science by hosting female high school students. The proposed instrumentation will complement and expand the capabilities of SIO researchers, as well as invited outside researchers. Seminars will be held for either the Marine Chemistry and Geochemistry or Marine Biology seminar series demonstrating the facility capabilities and results to the larger SIO populace