Engineering the synthesis of natural and non-natural anthocyanins in Escherichia coli

CBET-0756601 Koffas知识价值该项目的目标是开发用于合成多酚（如花青素）的天然和非天然化学结构的方法，这些多酚可用作染料，但也具有潜在的营养和药物应用。随着花青素苷的生物合成、应用和生化机制的明确，对标准化合成方法的需求变得越来越重要，该方法可以有效地和高纯度地生产任何天然花青素苷以及非天然花青素苷衍生物。这种合成将利用有机合成和代谢工程的进步，为潜在的数千种模拟天然花青素化学结构的化合物创造独特的合成平台。这些不同技术的融合使用将使人们能够合成高丰度的常见、稀有和非天然化合物，并有助于在精心控制的研究中了解植物多酚对健康的有益影响。更广泛的影响：更广泛的影响将通过积极的宣传活动产生，旨在提高公众对生物工程所提供的巨大机会和前景的认识。通过继续参加由计算研究中心组织的夏季研讨会，首席研究员（PI）将为广大高中教师和学生提供独特的教育体验。此外，通过PI继续参与布法罗大学旨在招募少数民族高中生参加暑期实习的各种方案，该项目将为少数民族和经济弱势群体提供指导机会。

CBET-0756601KoffasIntellectual Merit The goal of this project is to develop methodologies for the synthesis of natural and non-natural chemical structures of polyphenols such as anthocyanins that can be used as dyes but also have potential nutraceutical and pharmaceutical applications. As the biosynthesis, applications and biochemical mechanisms of the anthocyanins gain definition, the need for standardized synthetic processes that can produce any natural anthocyanin as well as non-natural anthocyanin derivatives efficiently and in high purity gains in importance. Such syntheses would take advantage of advances in organic synthesis and metabolic engineering in order to create unique synthetic platforms for potentially thousands of compounds that mimic the natural anthocyanin chemical structures. The convergent use of these disparate technologies will enable one to synthesize common, rare and non-natural compounds in high abundance and help understand the beneficial health effects of plant polyphenols in carefully controlled studies.Broader Impact: Broader impact will result through an aggressive outreach activity aimed at increasing the awareness of the general public on the vast opportunities and promise bioengineering has to offer. By continuing participation in summer workshops organized by the Center for Computational Research, the Principal Investigator (PI) will provide a unique educational experience to a wide pool of high-school teachers and students. In addition, through the PI's continuing participation in various programs at the University of Buffalo aimed at recruiting minority high-school students for summer internships, this project will offer mentoring opportunities for minorities and economically disadvantaged groups.