Nanoparticles Enabled Dual-Fluorescence Modulation: A New Method for Reliably Detecting Biomolecular Recognitions

化学系的分析和表面化学(ASC)计划将支持华盛顿州立大学的Alexander Li教授的研究计划。这个项目的实质是设计和合成新型的聚合物纳米颗粒，其红色荧光信号可以通过外部光脉冲进行调制，使其具有幅度和相位。当纳米颗粒识别与绿色荧光供体相关的分析物时，荧光共振能量转移(FRET)将把调制从纳米颗粒传播到绿色荧光供体，绿色荧光供体的信号将以相同的频率进行调制，明确确认相互作用。这项研究将提供一种新的分析方法，显著提高荧光检测的统计可信度，包括荧光成像、荧光传感器和基于荧光的定量分析。它将为研究生和博士后实习生提供在定量生物分析中使用先进光谱方法的极好的教育培训机会。

The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry will support the research program of Prof. Alexander Li of Washington State University. The essence of this project is to design and synthesize novel polymer nanoparticles, whose red-fluorescence signal can be modulated using external light pulses so that it has amplitude and a phase. When the nanoparticle recognizes the analyte associated with a green-fluorescence donor, fluorescence resonance energy transfer (FRET) will propagate the modulation from the nanoparticle to the green fluorescence donor, whose signal will be in turn modulated at the same frequency, unambiguously confirming interactions. This study will provide a new analytical method that dramatically improves the statistical confidence of fluorescence detection, including fluorescence imaging, fluorescence sensors and fluorescence-based quantitative analyses. It will provide excellent educational training opportunities for graduate students and postdoctoral trainees in the use of advanced spectroscopic methods in quantitative bioassays.