Systems Approach to Dynamic Atomic Force Microscopy

本项目的目标是建立一个基于模型的同步地形和幅度调制原子力显微镜(AFM)参数估计的框架。将开发适合实时识别的尖端-样品相互作用的参数模型。谐波平衡和功率平衡工具将被结合到模型参数的估计中。基于该模型的幅值和相位动力学将被用来用实验数据验证该模型，并随后用于控制设计目的。这些方法将被用于研究酵母细胞。将研究针尖-样品相互作用势的非参数重建框架。关于幅度调制原子力显微镜如何能够很好地破译不同的样品相互作用的限制将被研究。国际和平研究所将通过互访学生和举办专门讲习班，继续推动物理界和工程界之间技术知识的协同转让。将开发一种基于网络的AFM远程操作，并将其整合到一门关于纳米审讯的课程中。微型悬臂正被用于各种领域，影响越来越大，并对科学产生了根本性的影响。事实上，它们为纳米技术提供了巨大的推动力。本项目研究的幅度调制原子力显微镜方法是迄今为止使用最多的原子力显微镜模式，因为它可以对样品进行原子分辨率的成像，并且是非侵入性的，因此对于生物技术相关的研究特别有用。这个项目中使用的方法是可行的，因此将为研究基础科学打开大门。PI与领先的生物学相关扫描探针显微镜公司有很强的合作关系。这一合作有望促进在该项目中开发的理论和技术在PI的学术机构和SPM行业之间的转移。根据这一提议开发的基于网络的原子力显微镜远程操作将有助于激发人们对科学的兴趣，并将形成一种教学工具。

The goal of this project is to establish a framework for model based simultaneous topography and parameter estimation in the amplitude modulation atomic force microscopy (AFM). Parametric models of tip-sample interaction that are amenable to real-time identification will be developed. Harmonic balance and power balance tools will be incorporated towards the estimation of the model parameters. The amplitude and phase dynamics based on the model will be developed, which will be used to validate the model with experimental data and subsequently used for control design purposes. These methods will be used to study yeast cells. A framework for non-parametric reconstruction of tip-sample interaction potential will be researched. Limitations on how well amplitude modulated AFM can decipher different sample interactions will be studied. The PI will continue to push the synergistic transfer of know-how between the physics and engineering communities by exchanging student visits and presenting specialized workshops. A web based remote operation of the AFM will be developed and integrated into a course on nano-interrogation.Micro-cantilevers are being used in diverse areas with increasing impact and have influenced science in a fundamental manner. Indeed, they provided a significant impetus to nanotechnology. The amplitude modulation atomic force microscopy method to be researched in this project is by far is the most used AFM mode as it can image samples with atomic resolution and is non-invasive and is thus particularly useful for biotechnology related investigation. The methods to be used in this project are enabling and therefore will open doors for investigating basic science. The PI has strong collaboration with leading biology related scanning probe microscope companies. This collaboration is expected to foster transfer of the theory and technology developed in this program between the academic institution of the PI and the SPM industry. The web-based remote operation of AFMs to be developed under this proposal will help excite interest in science and will form an instructional tool.