Arabidopsis 2010: Functional Genomics of NBS-LRR Mediated Resistance

建议编号IOS-0822393该项目融合了遗传、进化和功能方法，以在分子水平上了解植物和病原体的共同进化。将分析酵母和植物中的蛋白质-蛋白质相互作用，以建立一个由受体(NBS-LRR)蛋白质、植物信号成分和细菌和卵菌病原体的毒力效应蛋白质组成的相互作用网络。这些相互作用对疾病的功能意义将通过各种细胞生物学和疾病检测来研究。该项目将揭示效应器的靶标是否代表植物中被多个效应器和病原体利用的有限数量的弱点，以及决定感染表型的蛋白质复合体是否共同进化。编码NBS-LRR蛋白及其结构域和信号蛋白的基因文库将通过拟南芥生物资源中心分发，这些基因是所研究的候选植物靶标。全球方法将产生大量数据，这些数据将影响许多致力于了解植物抗病分子基础的实验室，并有助于拟南芥众多基因的功能表征。该项目的数据将通过项目网站(http://niblrrs.ucdavis.edu)和拟南芥信息资源广泛传播。破译抗性的分子决定因素不仅具有根本的利益，而且具有重要的实际意义。对感知和反应机制的进一步了解将为开发抗病植物提供新的可能性。因此，该项目将为植物育种者提供更多的选择，以实现更持久的抗性。参与该项目的研究人员将受益于跨学科培训，其中包括防御反应组件的分子遗传学和细胞生物学，以及病原体-宿主相互作用中涉及的蛋白质-蛋白质相互作用。外展工作将包括通过课程开发、讲习班和实习将基因组学方法传播到高中。

Proposal number IOS-0822393This project fuses genetic, evolutionary, and functional approaches to understand the co-evolution of plants and pathogens at the molecular level. Protein-protein interactions will be analyzed in yeast and in plants to build an interaction network of receptor (NBS-LRR) proteins, plant signaling components, and virulence effector proteins from bacterial and oomycete pathogens. The functional significance of these interactions to disease will be studied by a variety of cell biology and disease assays. The project will reveal whether targets of effectors represent a limited number of points of weakness in the plant that are exploited by multiple effectors and pathogens and whether protein complexes that determine the infection phenotype co-evolve. Libraries of genes encoding NBS-LRR proteins and their domains as well as signaling proteins that are candidate plant targets of effectors studied will be distributed through the Arabidopsis Biological Resource Center.The global approach will generate large amounts of data that will impact many labs focused on understanding the molecular basis of disease resistance in plants as well as contribute to the functional characterization of numerous genes in Arabidopsis. Data from the project will be broadly disseminated through the project web site (http://niblrrs.ucdavis.edu ) and through The Arabidopsis Information Resource. Deciphering the molecular determinants of resistance has practical importance as well as fundamental interest. A greater understanding of the mechanisms of perception and responses will provide new possibilities for developing disease resistant plants. Consequently, this project will expand the options available to plant breeders to achieve more durable resistance. Researchers involved in the project will benefit from inter-disciplinary training that includes the molecular genetics and cell biology of the defense response components and protein-protein interactions involved in pathogen-host interactions. Outreach efforts will include dissemination of genomics approaches to high schools through curriculum development, workshops and internships.