U.S.-U.K. Planning Visit: Biochemical characterization of a candidate plant UV-B photoreceptor

Oise 08-36098 Ann Stapleton北卡罗来纳大学威尔明顿分校这是一次计划中的访问，为PI和她的一名本科生前往英国卡迪夫大学旅行提供支持。美国首席研究员是北卡罗来纳大学威尔明顿分校的安·斯台普顿。外国合作者是光化学和生物化学专家Gerlad Richter。这次访问的主要目的是在国际和平协会和里希特教授之间建立长期的合作伙伴关系。他们的研究重点是紫外线-B(UV-B)光感受器。尽管世界各地的科学家进行了密集的努力，但尚未发现UV-B光感受器。拟南芥候选蛋白的突变特征为进一步的生化分析提供了有趣的机制假说。第一个UV-B光感受器的生化特征对于理解臭氧消耗效应是关键，例如预测随着UV-B增加而营养水平改变的潜在程度。总之，研究人员将订购一种密码子使用优化的合成基因，用于在大肠杆菌中表达。

OISE 08-36098Ann StapletonUniversity of North Carolina - WilmingtonThis is a planning visit that provides support for the PI and one of her undergraduate students to travel to Cardiff University in the United Kingdom. The US principal investigator is Ann Stapleton from the University of North Carolina at Wilmington. The foreign collaborator is Gerlad Richter, an expert in photochemistry and biochemistry. The primary purpose of this visit is to establish a long-term collaborative partnership between the PI and Professor Richter. The focus of their research is on ultraviolet-B (UV-B) photoreceptors. No UV-B photoreceptor has been identified despite intensive effort by scientists world-wide. Mutant characterization of a candidate protein from Arabidopsis thaliana has provided intriguing mechanistic hypotheses for further biochemical analysis. Biochemical characterization of the first UV-B photoreceptor is key to understanding ozone depletion effects, such as prediction of the potential extent of trophic level alterations with increased UV-B. Together, the researchers will order a synthetic gene with optimized codon usage for E. coli expression.