Analysis of ESCRT-Dependent Trafficking Mechnisms in Arabidopsis

知识价值。本项目旨在了解控制植物内体介导的质膜蛋白降解的分子机制，以及依赖于内体运输途径的细胞和发育过程。在所有真核细胞中，内体室在膜蛋白的分类、回收和周转、受体的下调以及蛋白质向液泡和溶酶体的运输中发挥着重要作用。本项目重点研究两种重要的植物内体蛋白SKD1和CHMP1，它们与其他内体蛋白复合物协同作用，调节膜蛋白的降解分选。内体运输在植物发育、细胞类型规范、细胞壁重塑、向地性反应、信号转导和激素运输等多个方面发挥着重要作用。本文的研究将为植物细胞生物学领域提供有价值的信息。更广泛的影响。该项目将对教育和外联活动产生重要影响。首席研究员目前在威斯康星大学教授本科课程。该计划的另一部分是招募一名或多名研究生，另外还有一名已经在实验室工作的研究生，以研究本研究计划的中心主题。将适当考虑从代表性不足的群体中征聘个人。新成像技术的发展对教育计划也很重要，因为PI致力于为校园内的本科生和研究生开发细胞生物学和细胞成像研讨会，作为威斯康星大学植物学系新成像中心教育活动的一部分。该中心最初由美国国家科学基金会资助。

Intellectual merit. This project aims to understand the molecular machinery that controls endosomal-mediated degradation of plasma membrane proteins in plants as well as the cellular and developmental processes that depend on endosomal trafficking pathways. Endosomal compartments play a fundamental role in the sorting, recycling, and turnover of membrane proteins, the down-regulation of receptors, and the trafficking of proteins to vacuoles and lysosomes in all eukaryotic cells. This project focuses on two important plant endosomal proteins, SKD1 and CHMP1, which work in coordination with other endosomal protein complexes to regulate sorting of membrane proteins for degradation. Endosomal trafficking plays a role in various aspects of the plant life, such as development, cell type specification, cell wall remodeling, gravitropic response, signal transduction, and hormone transport. The research proposed here will provide valuable information in this plant cell biology area.Broader impacts. The project will have important impacts on education and outreach. The Principal Investigator is currently teaching undergraduate courses at the University of Wisconsin. An additional part of the plan is to recruit one or more graduate students, in addition one already in the laboratory, to work on the central topic of this research proposal. Due consideration will be given to recruitment of individuals from under-represented groups. The development of new imaging techniques will also be important for the educational plan, since the PI is committed to develop workshops on cell biology and cell imaging for undergraduate and graduate students across campus as part of the educational activities of the new Imaging Center in the Department of Botany-University of Wisconsin. The Center was initially supported by NSF funds.