Directed Evolution of Specific Cell Penetrating Peptides

该奖项是根据2009年《美国复苏和再投资法案》(公法111-5)资助的。定向进化技术为设计新的多肽提供了巨大的潜力，但这种方法的关键挑战是开发合适的选择程序。PI将使用基于噬菌体展示技术的新型双功能平台，并将与生理相关的选择协议相结合。一旦优化，这个平台将允许人们快速进化出有价值的新的SCPP序列。广泛的影响这个项目产生的更广泛的影响来自新的实验操作和治疗选择，将使新的SCPP成为可能。最近的研究工作已经产生了许多令人兴奋的基于机制的疗法。然而，将这些疗法提供给他们的目标一直是一个重大的限制。新的SCPP将能够扩大这些毁灭性疾病的潜在治疗手段。这个跨学科的研究项目还将为哥伦比亚大学和周围社区的新工程师的教学、培训和指导提供一个肥沃的环境。

This award is funded under the American Recovery and Reinvestment Act of 2009(Public Law 111-5).0853946Banta Intellectual merit The intellectual merit of this proposal results from the first demonstration of the directed evolution of specific cell penetrating peptides (SCPPs). The directed evolution technique offers tremendous potential for the engineering of new peptides, but the critical challenge in this approach is the development of appropriate selection procedures. The PIs will use a novel bi-functional platform based on phage display technology, and this will be coupled with a physiologically relevant selection protocol. Once optimized, this platform will allow one to rapidly evolve valuable new SCPP sequences.Broader impacts The broader impacts of this project arise from the new experimental manipulations and therapeutic treatment options that will be made possible by the novel SCPPs. Recent research efforts have produced many exciting mechanism-based therapies. However, the delivery of these therapies to their targets has been a significant limitation. The new SCPPs will enable the expansion of the armamentarium of potential therapies for these devastating diseases. This interdisciplinary research project will also provide a fertile environment for the teaching, training, and mentoring of new engineers both at Columbia University and in the surrounding community.