A Mechanically Based Polymer Microfiber Approach to Probe Mechanotransduction in Calcium Response of Stem Cells

题目：一种基于机械的聚合物微纤维方法来探测干细胞钙反应中的机械转导PI: Philip LeDuc，卡内基梅隆大学探测活细胞对机械刺激的反应的能力与基于材料的技术直接相关。虽然没有一种独特的技术可以完美地模仿生理环境。也有人提出，最佳的组织恢复与细胞的力学密切相关，通过建立基于新材料的技术可以实现基本的理解。此外，干细胞已被用于治疗基于力学的系统，如心脏病，但相关恢复过程的机制仍存在争议。该项目将开发一种基于材料的方法来研究干细胞的生物力学和相关的活细胞钙反应。该项目将在材料力学、成像和细胞力学等领域取得重要成果。通过为未来的研究人员提供优化干细胞治疗的机械刺激的能力，该项目将具有变革性，这可能最终有助于医疗应用的成功，包括心脏治疗和组织工程，可能挽救和改善数百万人的生命。该项目还将建立一个教育和培训管道，为未来的工程和科学领导者做好准备。这将通过与K-12学生、本科生和研究生的合作来完成。这些努力将包括宾夕法尼亚州学术上最具挑战性的公立学校之一，那里的少数民族人口超过95%。这项工作还将与斯隆基金会少数族裔博士项目相结合，继续建立多元化的努力。

TITLE: A Mechanically Based Polymer Microfiber Approach to Probe Mechanotransduction in Calcium Response of Stem Cells PI: Philip LeDuc, Carnegie Mellon UniversityThe ability to probe the response of living cells to mechanical stimulation is directly tied to material-based technology. There is though no one unique technology, which can imitate the physiological environment perfectly. It has also been suggested that optimal tissue recovery is vitally related to the mechanics of cells and that essential understanding can be achieved through building new materials based technology. Furthermore, stem cells have been used in therapy for mechanics based systems such as in cardiac disease, but the mechanisms of the associated recovery process are debated. This project will develop a material-based approach to investigate the biomechanics of stem cells and associated live-cell calcium response. This project will have important results in areas including mechanics of materials, imaging, and cell mechanics.This project will be transformative through providing future researchers with the ability to optimize mechanical stimulation for stem cell therapy, which may ultimately contribute to successes in medical applications including cardiac therapies and tissue engineering that could potentially save and make better millions of lives. The project also will build an education and training pipeline for preparing future leaders in engineering and science. This will be accomplished through work with K-12 students, undergraduates, and graduate students. These efforts will include one of the most academically challenged public schools in Pennsylvania, whose minority population is greater than 95%. This work will also be integrated with the Sloan Foundation Minority PhD program in continuing to build diversity efforts.