Advancing Genome-Enabled Tools: Guiding Inoculum Design for Sulfate-Reducing Mine Drainage Treatment Systems

科罗拉多州立大学高级基因组启用工具：硫酸盐还原矿井排水处理系统的免疫设计本项目的目标是证明基因组启用工具可以为接种剂的设计提供有价值的指导，以提高生物处理系统的性能和可靠性。这项研究的重点将集中在硫酸盐还原渗透反应区用于矿井排水处理，但该方法预计将广泛适用于任何生物处理系统。本研究的具体目标是：1-比较不同接种产生的群落关于硫酸盐还原活性和对压力的响应2-比较基于以上信息设计的接种产生的群落关于硫酸盐还原活性和对胁迫的响应，3-建立统计模型以确定控制和预测性能的关键参数。将使用不同环境来源和纯培养的组合接种柱子来实现这些目标。将战略性地应用一套基因组使能工具，包括16S rRNA和16S rRNA基因的毛细管电泳单链构象多态性，针对16S rRNA基因和关键群体功能基因的定量聚合酶链式反应，以及特定功能基因的克隆和测序。重点将是确定针对社区结构变量和功能变量的工具的相对效用。

ABSTRACTCBET 0651947Amy J. Pruden and Kenneth F. ReardonColorado State UniversityAdvancing Genome-Enabled Tools: Guiding Inoculum Designfor Sulfate-Reducing Mine-Drainage Treatment SystemsThe goal of this project is to demonstrate that genome-enabled tools can provide valuable guidance for the design of inocula in order to improve the performance and reliability of biological treatment systems. The focus of this study will be on sulfate-reducing permeable reactive zones employed for mine drainage treatment, but the approach is expected to be broadly applicable to any biological treatment system.The specific aims of this study are:1- compare communities resulting from different inocula with respect to sulfate-reducing activity and response to stress2- compare communities resulting from inocula that have been designed based on information from above with respect to sulfate-reducing activity and response to stress, and 3- develop statistical models in order to identify critical parameters for controlling and predicting performance.Columns inoculated with various combinations of environmental sources and pure cultures will be used to accomplish these aims. A suite of genome-enabled tools will be strategically applied, including capillary electrophoresis single strand conformation polymorphism of 16S rRNA and 16S rRNA genes, quantitative polymerase chain reaction targeting 16S rRNA genes and functional genes of key groups, and cloning and sequencing of specific functional genes. An emphasis will be on determining the relative utility of tools targeting community structural versus functional variables.