RUI: Novel Mobile Phase Additives for Reversed-phase Liquid Chromatography

分析和表面化学项目支持南阿拉巴马大学的Jason Coym教授在反相液相色谱（RPLC）中探索不同选择性和保留的新型添加剂（如胆固醇）。RPLC可能是最常用的液相色谱形式，是分离溶液中分析物的关键方法，在制药和生化设置中大量使用。这项工作需要动态地将胆固醇涂在固定相上，作为一种更简单、更灵活的替代典型的共价连锁。一个平行的目标是使用胆固醇增强和脂质包被相作为生物分割研究的膜模拟物。独特的色谱选择性的发展，以及容易调节选择性的能力，是分析和生物分析化学家的广泛兴趣。参与本项目的学生将获得色谱、物理化学和生物化学的知识和实践，从而认识到化学各学科之间的相互依存关系。

The Analytical and Surface Chemistry Program supports Professor Jason Coym of the University of South Alabama to explore novel additives (e.g. cholesterol) for varying selectivity and retention in reversed-phase liquid chromatography (RPLC). Perhaps the most commonly used form of liquid chromatography, RPLC is a key method for separating analytes in solution, and is used heavily in pharmaceutical and biochemical settings. The work entails dynamically coating cholesterol onto the stationary phase, as a simpler and more flexible alternative to typical covalent linkage. A parallel aim is to use cholesterol-enhanced and lipid-coated phases as membrane mimics for studies of biopartitioning.The development of unique chromatographic selectivities, and the ability to easily modulate selectivity, is of broad interest to analytical and bioanalytical chemists. Students working on this project will gain knowledge and practice working with chromatography, physical chemistry, and biochemistry, thereby coming to realize the interdependence of the various disciplines of chemistry.