BYPASS1 root-to-shoot signaling: the mobile signal interacts with the auxin pathway

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助。SieburthIOS 0922288 BYPASS 1根到茎的信号传导：移动的信号与生长素途径相互作用植物以综合的方式发育，即使根和茎系统在空间上是分开的，并且感知不同的环境条件。 例如，根直接检测土壤压实或干旱，但不利的条件也会导致芽发育的改变。 生理学研究表明，根产生的信号分子移动到芽，但这些信号分子如何修改芽的发展是未知的。 本项目将研究如何通过拟南芥bypass 1突变体中过量产生的根源长距离信号来调节芽的发育。 这种源自根的物质阻止叶的发育。 最近的数据表明，生长限制是通过减少对生长素（一种重要的植物激素）的反应而发生的。 研究人员将使用分子和生理学方法来研究激素反应。 生长素反应途径的具体步骤将使用遗传和生物化学工具进行分析。 这项研究计划将揭示机理的细节根源信号与生长素的相互作用。 限制新梢生长的根源信号使农业生产严重减少。 了解根源信号如何调节芽发育程序可能会导致替代农业实践，最大限度地提高生产力。 这项工作的更广泛影响是科学的（推进我们对信号通路的知识，通过出版物和会议上的演讲分享）和教育。 两名本科生，其中一名来自服务不足的背景，将接受遗传学和分子生物学方面的培训。 1名博士后将接受传播和教学培训。 博士后和本科生将共同开发一个本科生发育生物学类实验室练习。 本实验将提供长距离信令的实践经验，并将广泛传播。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Leslie E. SieburthIOS 0922288BYPASS1 root-to-shoot signaling: the mobile signal interacts with the auxin pathwayPlants develop in an integrated manner, even though root and shoot systems are separated spatially and perceive different environmental conditions. For example, roots directly detect the soil compaction or drought, but unfavorable conditions also lead to altered shoot development. Physiological studies have shown that roots produce signaling molecules that move to the shoot, but how these signaling molecules modify shoot development is unknown. This project will investigate how shoot development is modulated by a root-derived long distance signal that is over-produced in Arabidopsis bypass1 mutants. This root-derived substance arrests leaf development. Recent data suggest that growth restriction occurs by reducing responses to auxin, an important plant hormone. The investigators will use molecular and physiological approaches to investigate hormone responses. Specific steps of the auxin response pathway will be analyzed using genetic and biochemical tools. This research program will reveal mechanistic detail of root-sourced signal interaction with auxin. Root-derived signals that limit shoot growth impose severe reductions in agriculture. Understanding of how shoot development programs are regulated by root-derived signals could lead to alternative agricultural practices that maximize productivity. Broader impacts of this work are scientific (advancing our knowledge of signaling pathways, to be shared through publications and talks at conferences) and educational. Two undergraduate students, one from an under-served background, will be trained in genetics and molecular biology. One postdoctoral scholar will receive communication and teaching training. The postdoc and undergraduates will work together to develop a laboratory exercise for an undergraduate developmental biology class. This lab will provide hands-on experience with long distance signaling, and will be disseminated widely.