MRI: Acquisition of an Ultra High Performance Liquid-Chromatography Tandem Mass-Spectrometer for a Multi-user Core Mass-Spectrometry Facility

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。南达科他州立大学化学与生物化学系将获得一台高性能液相色谱串联质谱仪，用于小分子和大生物分子的高级分析。这将支持正在进行的和拟议的跨学科研究项目，包括化学、生物化学、药学、生物学和微生物学，以及校园其他几个部门的其他研究项目。它将用于一系列广泛的研究项目，包括分析格陵兰冰芯的高氯酸盐，以确定环境中高氯酸盐的自然和人为来源；鉴定复杂混合物中的蛋白质，包括蛋白质-蛋白质相互作用的研究；鉴定重要疾病的生物标志物，确定对特定生物现象至关重要的蛋白质。其他项目包括化学战剂暴露分析、细菌生物工程以及与文化和生活方式实践有关的化学暴露差异调查。它将用于一项水分析推广项目，该项目将利用网络技术将所有层次的教育（K-12、本科、研究生和教师）联系起来。此次收购还将为一些全州范围的合作和研究中心提供支持。质谱法（MS）是通过测量样品中分子组分电离后经质谱仪检测后的质量，来鉴定样品的化学成分并确定其纯度的方法。色谱法是一种先于质谱分析的分离技术。它将混合物分离成几种化学成分，然后用质谱仪进行分析。这些是广泛使用的分析技术，用于鉴定和定量样品的化学成分。对学生进行使用现代仪器的技术训练是很重要的。这些仪器将成为教学和研究的一个组成部分。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With this award from the Major Research Instrumentation (MRI) program, the Department of Chemistry and Biochemistry at South Dakota State will acquire an ultra high performance liquid-chromatography tandem mass-spectrometer for advanced analysis of small molecules and large bio-molecules. This will support ongoing and proposed interdisciplinary research projects in chemistry, biochemistry, pharmacy, biology and microbiology, as well as other research projects from several other departments on campus. It will be used in a broad range of research projects including, analysis of perchlorate from Greenland ice cores to determine natural and anthropogenic sources of perchlorate in the environment; identification of proteins in complex mixtures, including investigations of protein-protein interactions; identification of biomarkers of important diseases, and determination of proteins of key importance to specific biological phenomena. Other projects include analysis of chemical warfare agent exposure, bioengineering of bacteria, and investigation of differences in chemical exposure related to cultural and lifestyle practices. It will be used in a water analysis outreach project that connects all levels of education (K-12, undergraduate, graduate, and faculty) with the help of cyber-enabled technology. This acquisition will also provide support to a number of state-wide collaborations and research centers.Mass spectrometry (MS) is used to identify the chemical composition of a sample and determine its purity by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. Chromatography is an isolation technique that precedes the mass spectrometry analysis. It separates a mixture into its several constituent chemicals which are then analyzed by the mass spectrometer. These are widely used analytical techniques to identify and quantify the chemical composition of a sample. It is important for students to be trained in the technique using modern instrumentation. These instruments will be an integral part of teaching as well as research.