MRI: Acquisition of a Surface Plasmon Resonance Instrument

该奖项是根据2009年《美国复苏和再投资法案》(Public Law 111-5)资助的。在重大研究仪器(MRI)项目的这一奖励下，Arne Gericke教授和他的同事Edgar A.Kooijman将获得一台表面等离子体共振(SPR)仪器。研究的目的包括：1)脂类介导的蛋白质功能；2)可交换的脂蛋白和脂滴蛋白-脂相互作用；3)雄激素调节激素反应基因的研究；4)丙泊酚/PKC相互作用的研究；5)蛋白磷酸酶1-γ2与抑制剂相互作用的特征；以及5)DNA和RNA四链形成的特征。在一种新的方法中，计划在SPR芯片表面组装有序介孔有机硅(OMOS)，以开发可用于传感应用或环境修复的OMO。SPR生物传感器是用于研究生物分子相互作用的分析仪器。SPR生物传感器测量靠近表面的溶液的折射率变化。这些变化可能是由于分析物与表面结合的配体结合时发生的浓度变化引起的。随着时间的推移，变化可以反映关联和分离，从而反映亲和力。这个仪器将供本科生在研究中使用。它还将用于肯特州立大学化学、物理和生物部门联合开发的生物光子学跨学科课程，以改善这一重要科学领域的本科生在进入劳动力大军或研究生院之前的教育。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With this award from the Major Research Instrumentation (MRI) program, Professor Arne Gericke and his colleague Edgar A. Kooijman will acquire a surface plasmon resonance (SPR) instrument. The research uses include projects aimed at studying: 1) lipid mediated protein functions; 2) exchangeable apolipo- and lipid droplet protein-lipid interactions; 3) the investigation of androgen regulation of hormone responsive genes; 4) the investigation of propofol/PKC interaction; 5) the characterization of protein phosphatase 1 gamma 2 interaction with inhibitors; and 5) the characterization of DNA and RNA quadruplex formation. In a novel approach, it is planned to assemble ordered mesoporous organosilicas (OMOs) on an SPR chip surface to develop OMOs that can be used for sensing applications or in environmental remediation.SPR biosensors are analytical instruments used to study biomolecular interactions. An SPR biosensor measures changes in refractive index of a solution close to a surface. The changes can be caused by changes in concentration of an analyte that occur on binding to a surface-bound ligand. Changes over time can reflect association and dissociation and thus, affinity. This instrument will be used by undergraduates in their research. It will also be used in a biophotonics cross-disciplinary course under development jointly by Kent State's Chemistry, Physics and Biology departments to improve the education of undergraduate students in this vital scientific area prior to their entering the workforce or graduate school.