Acquisition of a Cell Lab Quanta SC Benchtop Flow Cytometer

[09:32 . 195]本提案的目的是获得一种低成本的台式流式细胞仪，以增加PI？在微流体细胞分离领域的研究活动。所需的仪器是贝克曼库尔特公司生产的Cell Lab Quanta SC系统。该系统具有基于荧光灯发射和三种不同波长检测的异质悬浮液中细胞计数的能力，以及测量3-40范围内细胞大小ìm的能力。Quanta SC系统能够对细胞大小和荧光进行准确、自动化的测量，这将极大地增强PI在以下方面的变变性研究工作：1)基于受体对流体剪切应力的反应，对细胞配体对进行指纹识别，以识别尚未发现的、存在于某些组织生态位中的识别干细胞或祖细胞群体；（ii）设计用于检测葡萄膜炎和眼内淋巴瘤的微流控免疫分型系统；（iii）系统地富集心肌细胞，作为有效设计心脏组织贴片的先导；（四）设计微流体细胞分离装置，分离和表征心脏祖细胞。通过东北大学现有的青年学者和RET项目参加暑期实验研究的高中学生和教师将有机会学习流式细胞术的基础知识，该仪器比传统的流式细胞术系统更容易获得和更容易使用。广达SC还将被整合到高级本科实验课程的微流体实验模块中。

0932195MurthyThe objective of this proposal is to acquire a low cost bench-top flow cytometer to augment the PI?s research activities in the area of microfluidic cell separation. The desired instrument is the Cell Lab Quanta SC system manufactured by Beckman Coulter Inc. This system has the ability to count cells in a heterogeneous suspension based on fluorescent light emission and detection at three distinct wavelengths as well as the ability to measure cell sizes in the range of 3-40 ìm. The capability to perform accurate, automated measurements of cell size and fluorescence with the Quanta SC system will greatly enhance the PI's transformative research efforts in (i) fingerprinting cell-ligand pairs based on receptor responses to fluid shear stress to identify as-yet undiscovered identify stem or progenitor populations that are resident in certain tissue niches; (ii) designing a microfluidic immunophenotyping system for the detection of uveitis and intraocular lymphoma; (iii) enriching cardiomyocytes systematically as a precursor to effectively designing cardiac tissue patches; and (iv) designing microfluidic cell separation devices to isolate and characterize cardiac progenitor cells. High school students and teachers participating in summer research at the experimental research via existing Young Scholar and RET programs at Northeastern University will have the opportunity to learn the fundamentals of flow cytometry with an instrument that is far more accessible and simpler to use than conventional flow cytometery systems. The Quanta SC will also be integrated into a laboratory module on microfluidics in a senior-level undergraduate laboratory course.