Designing carbohydrate nanoparticles for prolonged efficacy of bacteriocin against food pathogens

成功控制李斯特菌和沙门氏菌等食品病原体需要开发新的方法。新兴的食品纳米载体保证了受控抗菌剂递送的巨大潜力，以降低病原体污染的风险。从长远来看，基于纳米颗粒的抗菌剂递送方法可以补充或部分取代基于薄膜的活性包装。在这个项目中，目标是建立一种新的策略，通过碳水化合物纳米颗粒介导的细菌素传递来降低食品中病原体污染的风险。将单核细胞增生李斯特菌和鼠伤寒沙门氏菌分别用作革兰氏阳性和革兰氏阴性细菌模型。Nisin是FDA批准的一种有效的食品级抗菌肽，将被用作细菌素模型。植物糖原是一种食物来源的树枝状碳水化合物，将用于制备水溶性功能性纳米载体，能够吸收乳酸链球菌素用于控制递送。更广泛的影响本项目的实施将把纳米技术与国家食品安全和保障相结合，并为食品化学、食品微生物学和食品加工领域的前沿概念和实践创造新的教育机会。这项研究的结果将有助于减少李斯特菌和沙门氏菌等病原体对食品的污染，并可能有助于挽救生命。除了食品领域，这项研究产生的知识将有利于其他领域，如药物和营养品的控制输送。为了实现教育目标，将在高中、大学和工业一级大力开展指导和培训。该项目将为参与的本科生和研究生以及数百名参加由普渡大学惠斯勒碳水化合物研究中心主办的碳水化合物短期课程的工业人员提供宝贵的培训机会。此外，PI已与当地一所高中建立了关系，指导学生进行科学研究课程。该项目将为这些学生提供在真实的世界中工作和向真实的科学家学习的机会。为了确保该项目对代表性不足的群体的好处，PI将与普渡大学管理部门密切合作，以确定和招募少数民族本科生和研究生，以纳入拟议的研究。

Intellectual merit Successful control of food pathogens such as Listeria and Salmonella requires the development of new methodologies. Emerging food nanocarriers warrant great potentials for controlled antimicrobial delivery to reduce the risk of pathogen contamination. In the long run, the methodology of nanoparticle-based delivery of antimicrobials may complement or partially replace film-based active packaging. In this project, the goal is to establish a novel strategy to reduce the risk of pathogen contamination in food through carbohydrate nanoparticle-mediated delivery of bacteriocin. Listeria monocytogenes and Salmonella Typhimurium will be used as the Gram-positive and Gram-negative bacteria models, respectively. Nisin, an FDA approved potent food-grade antibacterial peptide, will be used as bacteriocin model. Phytoglycogen, a food-based dendritic carbohydrate, will be used to prepare water-soluble functional nanocarriers that are able to absorb nisin for controlled delivery. Broader impactsThe implementation of this project will integrate nanotechnology with national food safety and security, and create new education opportunities for cutting-edge concepts and practices in food chemistry, food microbiology, and food processing. The outcome of this research will contribute to the reduction of food contamination by pathogens such as Listeria and Salmonella and may help to save lives. Beyond the food area, the knowledge generated from this research will benefit other fields such as the controlled delivery of drugs and nutraceuticals. In an effort to accomplish educational goals, great efforts will be taken for mentoring and training at the high school, college, and industrial levels. This project will provide precious training opportunities for undergraduate and graduate students involved as well as for hundreds of industrial personnel attending the Carbohydrate Short Course hosted by the Whistler Center for Carbohydrate Research at Purdue. In addition, the PIs have established a relationship with a local high school for mentoring students in their Science Research course. This project will provide opportunities for these students to work in the real world and to learn from real scientists. To ensure the benefit of this project to underrepresented groups, the PIs will collaborate closely with Purdue administration to identify and recruit minority undergraduate and graduate students for inclusion in the proposed research.