SBIR Phase I: Detection and Determination of Viability of Cryptospordium Oocysts by Capillary Column Immunosensor

这个SBIR第一期项目将开发一种方法来检测100个卵囊/L或更低水平的隐孢子虫卵囊，并区分活的和不活的包囊。该团队将使用基于毛细管的免疫传感器的原型(Aegis 1000仪器)，结合加热和裂解处理来检测水中的隐孢子虫。该项目的更广泛/更广泛的商业影响将是毛细管免疫传感器的原型商业化，这将有可能扩大其在水行业的应用，用于活体水传播病原体检测。这将使自来水公司能够迅速应对和纠正微生物污染事件。

This SBIR Phase I project will develop a method to detect Cryptosporidium oocysts at a level of 100 oocysts/L or lower, and to differentiate between viable and non-viable cysts. The team will use a prototype of a capillary-based immunosensor (AEGIS 1000 instrument) coupled with heat and lysis treatment to detect Cryptosporidium in water.The broader/commercial impact of the project will be the commercialization of the prototype of capillary-based immunosensor, which has the potential to extend its application in the water industry for live waterborne pathogen detection. This will allow water utilities to promptly respond to and correct a microbial contamination event.