Physiological genomics of a polymorphic locus affecting dispersal and ecological dynamics

姓名（N）：马尔登，詹姆斯H.建议编号：IOS 0950416项目名称：影响扩散和生态动力学的多态性位点的生理基因组学本项目将研究琥珀酸脱氢酶d（sdhd）基因的等位基因变异如何影响作为集合种群生物学模式物种的昆虫的代谢和运动生理学。琥珀酸脱氢酶d（Sdhd）是编码线粒体酶SDH的亚基的核基因，其将TCA循环连接到电子传递链。除了其在代谢中的核心作用，SDH影响控制昆虫气管形态发生（和人类疾病，如血管化肿瘤）的缺氧诱导途径。在Melitaea cinxia蝴蝶中，初步数据显示Sdhd等位基因与SDH催化速率（Vmax）、飞行耐力、分散和建立新种群的能力以及已建立种群的人口统计学差异相关。通过揭示基因、发育、生理和生态动态之间的机制联系，这项研究将提供一个异常详细的生态和进化动态耦合的图景。数据将被用于模型，使更详细和预测性的了解影响物种的区域持久性的因素（这取决于集合种群的过程，包括扩散能力），并可以推广到研究遗传和生理变异的弹性，干扰和气候变化的作用。这项研究是与赫尔辛基大学正在进行的合作的一部分。 学生将获得进行国际合作科学的经验，并将接受综合生物学的培训。 本研究开发的基因组学工具将促进和促进新的功能基因组学技术在生物、生态和进化生物学中的应用。

Name: Marden, James H.Proposal Number: IOS 0950416Project Title: Physiological Genomics of a Polymorphism Locus Affecting Dispersal and Ecological DynamicsThis project will examine how allelic variation in the succinate dehydrogenase d (sdhd) gene affects metabolism and locomotion physiology of an insect that is a model species for metapopulation biology. Succinate dehydrogenase d (Sdhd) is a nuclear gene that encodes a subunit of the mitochondrial enzyme SDH, which links the TCA cycle to the electron transport chain. In addition to its central role in metabolism, SDH affects the hypoxia inducible pathway that controls insect tracheal morphogenesis (and human diseases such as vascularized tumors). In Melitaea cinxia butterflies, preliminary data show that Sdhd alleles are associated with differences in SDH catalytic rate (Vmax), flight endurance, ability to disperse and establish new populations, and demographics of established populations. By revealing the mechanistic links between genes, development, physiology, and ecological dynamics, this study will provide an unusually detailed picture of coupled ecological and evolutionary dynamics. Data will be used in models that enable a more detailed and predictive understanding of factors that affect regional persistence of species (which depends on metapopulation processes, including dispersal ability), and which can be generalized to examine the role of genetic and physiological variation in resiliency, disturbance, and climate change. The study is part of an ongoing collaboration with the University of Helsinki. Students will gain experience conducting international collaborative science and will receive training in integrative biology. Genomics tools developed in this research will promote and facilitate the application of new functional genomics technology to organismal, ecological, and evolutionary biology.