Conference: 2010 Protein Transport Across Cell Membranes GRC, Galveston, Texas

知识价值。本提案要求支持将于2010年3月7日至12日在德克萨斯州加尔维斯顿召开的2010年蛋白质跨细胞膜运输戈登研究会议（GRC）。蛋白质跨细胞膜转运GRC成立于2005年，旨在提供一个重点会议，将研究不同生物中蛋白质跨细胞膜转运或蛋白质插入细胞膜的研究科学家聚集在一起。在美国，没有其他定期召开的会议专门对这一研究领域进行深入报道。蛋白质转运机制的阐明仍然是现代细胞生物学的基本目标，因为对全测序基因组的分析表明，至少25%的细胞蛋白质通过细胞膜转运或插入细胞膜。此外，这些蛋白质转运机制的知识构成了理解进口缺陷的基础，在一些情况下，进口缺陷可能导致严重的疾病状态。细菌蛋白质转运机制也很重要，因为细菌特异性蛋白质转运途径负责毒素和毒力因子的分泌。本次会议的主要目标之一是促进使用不同模型系统和实验方法来研究蛋白质转运机制的科学家之间的互动。该领域的研究人员使用广泛的互补方法，包括细胞生物学，生化，遗传学，生物物理学，结构，计算和基因组分析，以提供对蛋白质运输和插入途径的新见解。蛋白质转运领域是国际化的，这反映在邀请演讲者和会议主席的选择上。更广泛的影响。这次蛋白质跨细胞膜转运GRC的首要目标是通过强烈鼓励研究生和博士后积极参与会议的各个方面，将研究，教育和培训结合起来。这次会议将使这些初级科学家接触到这一领域的主要实验室正在使用的广泛的实验系统和方法。在2005年和2007年的蛋白质跨细胞膜转运GRC会议上，研究生和博士后占与会者的40%。强烈鼓励会议申请者为海报会议提交摘要。将根据提交的海报会议摘要选择另外14位发言者。我们将尽一切努力确保为这些“突破性的演讲”挑选的演讲者包括高比例的研究生、博士后、初级教员和未被充分代表的少数民族成员。与会者将从申请者中挑选，目标是包括来自美国和其他国家的学术、工业和政府实验室的科学家。与会者将根据平权行动的原则从申请人中挑选。虽然会议的结果不会公布，但根据戈登研究会议的不记录政策，会议参与者将在正式会议和海报会议上进行高水平的信息交流。在2005年和2007年的会议上，几乎所有（90%）没有发言的人都展示了海报，所以基本上所有的参与者都以一种或另一种形式展示了他们的研究。加强青年科学家的参与是本次会议的一个重要目标，因为这将确保该研究领域在未来继续保持活力。

Intellectual merit. This proposal requests support for the 2010 Protein Transport across Cell Membranes Gordon Research Conference (GRC) to be convened March 7-12, 2010 in Galveston, Texas. The Protein Transport across Cell Membranes GRC was established in 2005 to provide a focused meeting that would bring together research scientists who investigate protein transport across or protein insertion into cellular membranes in diverse organisms. There is no other regularly scheduled meeting that occurs in the US that is devoted to an in-depth coverage of this research field. Elucidation of protein transport mechanisms remains a fundamental objective of modern cell biology, as the analysis of fully sequenced genomes indicates that at least 25% of cellular proteins are either transported across or inserted into cellular membranes. Furthermore, knowledge of these protein transport mechanisms forms the basis for understanding import defects, which in several cases can lead to severe disease states. Bacterial protein transport mechanisms are also important because bacterial-specific protein transport pathways are responsible for the secretion of toxins and virulence factors. One of the main objectives of this conference is to promote interactions between scientists who use different model systems and experimental approaches to investigate protein transport mechanisms. Researchers in this field use a broad range of complementary approaches including cell biological, biochemical, genetic, biophysical, structural, computational and genomic analysis to provide novel insight into protein transport and insertion pathways. The protein transport field is international, and this is reflected in the selection of invited speakers and session chairs. Broader impacts. The overarching objective of this GRC on Protein Transport across Cell Membranes is to integrate research, education and training by strongly encouraging the active participation of graduate students and postdoctoral fellows in all aspects of the conference. The conference will expose these junior scientists to a broad range of experimental systems and approaches that are being used by the leading laboratories in this field. Graduate students and postdoctoral fellows accounted for 40% of the attendees at the 2005 and 2007 meetings of the Protein Transport across Cell Membranes GRC. Conference applicants are strongly encouraged to submit abstracts for the poster sessions. Fourteen additional speakers will be selected based upon submitted poster session abstracts. Every effort will be made to insure that the speakers selected for these "late-breaking talks" include a high proportion of graduate students, postdoctoral fellows, junior faculty and members of under-represented minorities. Conference attendees will be selected from the pool of applicants, with the objective of including scientists from academic, industrial and government labs, both from within the US and other countries. The attendees will be selected from the pool of applicants in accord with the principles of affirmative action. Although results of the meeting will not be published, consistent with the off-the-record policy of the Gordon Research Conferences, a high level of exchange of information between conference participants at the formal sessions and poster sessions will occur. Almost all (90%) of non-speakers at the 2005 and 2007 meeting presented posters, so essentially all participants presented their research in one format or another. Enhancing the participation of junior scientists is a critical objective of this meeting, as this will insure the continued vitality of this research field in the future.