Nanofiber-Based Sensors for Oxygen Determination in Model Glioblastomas

1033991 Palmer拟议的研究将开发一种基于纳米纤维的传感器，能够量化迁移的胶质母细胞瘤人群及其周围的氧气分布。PI已经表明，电纺定向纤维阵列可以引导贴壁的人类来源肿瘤细胞的迁移。通过制造一种“核-壳”纳米纤维，氧敏感指示剂可以被添加到这种纤维的“核”上，同时“壳”保护细胞不受指示剂的影响。共聚焦显微镜可以询问氧含量，并使涉及一个或多个神经球的实验成为可能，它们如何与周围的[O2]以及彼此相互作用。一种传感器系统在人体内重建低氧、常氧和高氧状态，同时鼓励肿瘤转移已知的一个组成部分的迁移，将在许多人类癌症的研究中有用。此外，传感能力将使人们更好地理解在器官生成和替换过程中产生和整合新的正常人体组织时出现的类似问题。

1033991PalmerThe proposed research will develop a nanofiber-based sensor capable of quantifying oxygen distributions within and around populations of migrating glioblastoma populations. The PI has already shown that electrospun aligned fiber arrays can guide the migration of adherent human-derived tumor cells. By fabricating a "core-shell" nanofiber, oxygen-sensitive indicators can be added to the "core" of this fiber while the "shell" protects cells from exposure to the indicator. Confocal microscopy can interrogate oxygen content and enable experiments involving one or more neurospheres, how they interact with the surrounding [O2] and with each other. A sensor system that recreates hypoxic, normoxic, and hyperoxic conditions within the human body while simultaneously encouraging migration known to be a component of tumor metastasis will be useful in the study of many human cancers. Furthermore, the sensing capability will lead to increased understanding of similar issues that arise in the creation and integration of new, normal human tissue during organ generation and replacement.